Professor
Chemistry & Biochemistry

Steven Pascal

CHEMISTRY BUILDING 4501 ELKHORN AVE
NORFOLK, 23529

Ph.D. in Molecular Biophysics, Florida State University, (1993)

M.A. in Physics, Kent State University, (1987)

B.S. in , Nebraska Wesleyan University, (1985)

Contracts, Grants and Sponsored Research

Semler, B. and Pascal, S. "Synthesis of enterovirus positive-strand RNAs: discovery of viral and host determinants of RNP complex formation" $433,169. Federal. February 2020 - September 2023
"Biophysical characterization of full-length Par-4 tumor suppressor" $1,500. Old Dominion University. January 2019 - May 2019
"Par-4 tumor suppressor: structural changes that trigger the death of cancer cells" $10,000. Old Dominion University. January 2018 - May 2019
"Expression and Purification of HRV-14 proteins 3D & 3CD for in vitro studies" $1,500. Old Dominion University. January 2014 - May 2014
"Picornavirus RNA Template Synthesis, In Vitro Transcription and initial structural characterization" $1,500. Old Dominion University. January 2013 - May 2013
"Looking over a four-leaf clover: structural characterisation of a critical picornaviral RNA clover leaf and its interaction with a viral protein" $735,000. Foreign. January 2009 - January 2012
"The zipper, the butterfly and death: structural studies of an apoptosis-triggering protein-protein interaction linked to prostate cancer and neurodegenerative disease" $720,000. Federal. January 2006 - January 2010
"Biomedical Applications of Plant Genes" $83,000. January 2008 - January 2009
"Picornaviral Replication: Structures and Interactions of Two Key Viral Molecules" $630,000. Federal. January 2004 - January 2007
"Automated Tune and Match (ATM) Module for 700 MHz NMR Cryoprobe" $75,000. Federal. January 2005 - December 2005
"Structure and Interactions of a Protein Linked to Apoptosis Response in Prostate Tumors" $263,509. Federal. January 1998 - January 2001

Expertise

Chemistry
NMR, Structural Biology, Structure Prediction
Biology
Viruses, Cancer, Structural Biology, Structure and Function
Physics
Biophysics,SAXS,Bioinformatics

Research Interests

Combining NMR techniques with Small Angle X-ray Scattering (SAXS) and bioinformatics to determine protein and nucleic acid structure.

Articles

Raut, K. K., Pandey, S. M.., Kharel, G. M.. and Pascal, S. M.. Evidence of direct interaction between cisplatin and the caspase-cleaved prostate apoptosis response-4 tumor suppressor. Protein Science.
Pandey, S., Raut, K., Clark, A., Baudin, A., Djemri, L., Libich, D. S., Ponniah, K. and Pascal, S. (2023). Enhancing the conformational stability of the cl-Par-4 tumor suppressor via site-directed mutagenesis. Biomolecules 13 (4) , pp. 667-.
Raut, K. K., Ponniah, K. and Pascal, S. M. (2021). Structural Analysis of the cl-Par-4 Tumor Suppressor as a Function of Ionic Environment. Biomolecules 11 (386) , pp. 1-13.
Bowitch, A., Sahoo, A., Clark, A. M., Ntangka, C., Raut, K. K., Gollnick, P., Yu, M. C., Pascal, S. M., Walker, S. E. and Ferkey, D. M. (2021). Methylation of the D2 dopamine receptor affects binding with the human regulatory proteins Par-4 and Calmodulin. Micropublication Biology, Caltech Library.
Pascal, S., Garimella, R., Warden, M. S. and Ponniah, K. (2020). Structural biology of the enterovirus replication-linked 5'-clover leaf RNA and associated virus proteins. Microbiology and Molecular Biology Reviews 84(2).
Wortman, M., Dagdanova, A., Clark, A., Earl, G., Pascal, S., Johnson, E. and Dianne, D. (2020). A Synthetic Pur-based Peptide Binds and Alters G-quadruplex Secondary Structure Present in the Expanded RNA Repeat of C9orf72 ALS/FTD. BBA - Molecular Cell Research 1867.
Clark, A. M., Ponniah, K., Warden, M. S., Raitt, E. M., Smith, B. G. and Pascal, S. (2019). Tetramer formation by the caspase-activated fragment of the Par-4 tumor suppressor. FEBS Journal 286 , pp. 4060-4073.
Warden, M. S., Cai, K., Cornilescu, G., Burke, J. E., Ponniah, K., Butcher, S. E. and Pascal, S. (2019). Conformational Flexibility in the Enterovirs RNA Replication Platform. RNA 25 , pp. 376-387.
Clark, A. M., Ponniah, K., Warden, M. S., Raitt, E. M., Yawn, A. C. and Pascal, S. (2018). pH-induced Folding of the Caspase-cleaved Par-4 Tumor Suppressor: Evidence of Structure Outside of the Coiled Coil Domain. Biomolecules 8 (4) , pp. 162(17).
Abdelrasoul, M., Ponniah, K., Mao, A., Warden, M. S., Elhefnawy, W., Li, Y. and Pascal, S. (2017). Conformational Clusters of Phosphorylated Tyrosine. JACS (Journal of the American Chemical Society) 139 , pp. 17632-17638.
Warden, M., Tonelli, M., Cornilescu, G., Liu, D., Hopersberger, L., Ponniah, K. and Pascal, S. (2017). Structure of RNA stem loop B from the picornavirus replication platform. Biochemistry 56 (20) , pp. 2549-2557.
Sharp, J. A., Hair, P. S., Pallera, H. K., Kumar, P. S., Mauriello, C. T., Nyalwidhe, J. O., Phelps, C. A., Park, D., Thielens, N. M., Pascal, S. M., Chen, W., Duffy, D. M., Lattanzio, F. A., Cunnion, K. M. and Krishana, N. K. (2015). Peptide Inhibitor of Complement C1 (PIC1) Rapidly Inhibits Complement Activation after Intravascular Injection in Rats. PLOS.
Elhefnawy, W., Boudion, A., O’Saben, E., Abdelaal, M., Pascal, S. and Li, Y. (2015). Structural Analysis and Prediction of Protein Phosphorylation Sites. ISBRA 2015 , pp. 5.
Suzuki, R., Irie, R., Harntaweesup, Y., Tachibana, K., Holland, P. T., Harwood, D. T., Shi, F., Beuzenberg, V., Itoh, Y., Pascal, S. M., Edwards, P. B. and Satake, M. (2014). Brevisulcatic Acids, Marine Ladder-Frame Polyethers from the Red Tide Dinoflagellate Karenia brevisulcata in New Zealand. Organic Letters 16 , pp. 1580-1583.
Li, Q., Pascal, S. and Li, Y. (2014). Intrinsically Disorder Protein Prediction using Undersampling Feedforward Neural Networks and Predicted Amino Acid Features. Proceedings of Modeling, Simulation, and Visualization Capstone Conference.
Holland, P. T., Shi, F., Satake, M., Hamamoto, Y., Ito, E., Beuzenberg, V., McNabb, P., Munday, R., Briggs, L., Truman, P., Gooneratne, R., Edwards, P. and Pascal, S. M. (2012). Novel toxins produced by the dinoflagellate Karenia brevisulcata. Harmful Algae.
Venugopal, H., Edwards, P. JB., Schwalbe, M., Claridge, J. K., Libich, D. S., Stepper, J., Loo, T., Patchett, M. L., Norris, G. E. and Pascal, S. (2011). Structural, dynamic, and chemical characterization of a novel S-glycosylated bacteriocin. Biochemistry 50 (14) , pp. 2748–2755.
Sun, X., Jones, W. T., Harvey, D., Edwards, P. JB., Pascal, S., Kirk, C., Considine, T., Sheerin, D. J., Rakonjac, J., Oldfield, C. J. and others (2010). N-terminal domains of DELLA proteins are intrinsically unstructured in the absence of interaction with GID1/gibberellic acid receptors. Journal of Biological Chemistry 285 (15) , pp. 11557–11571.
Edwards, P. JB., Kakubayashi, M., Dykstra, R., Pascal, S. and Williams, M. AK. (2010). Rheo-NMR studies of an enzymatic reaction: evidence of a shear-stable macromolecular system. Biophysical journal 98 (9) , pp. 1986–1994.
Ponniah, K., Loo, T. S., Edwards, P. JB., Pascal, S., Jameson, G. B. and Norris, G. E. (2010). The production of soluble and correctly folded recombinant bovine $β$-lactoglobulin variants A and B in Escherichia coli for NMR studies. Protein expression and purification 70 (2) , pp. 283–289.
Heady, S. J., MacAskill, U. K., Wright, M. A., Claridge, J. K., Edwards, P. B., Farley, P. C., Christeller, J. T., Laing, W. A. and Pascal, S. M. (2010). The Solution structure of the squash aspartic acid proteinase inhibitor (SQAPI) and mutational analysis of pepsin inhibition. J.Biol.CHem.
Schwalbe, M., Dutta, K., Libich, D. S., Venugopal, H., Claridge, J. K., Gell, D. A., Mackay, J. P., Edwards, P. B. and Pascal, S. M. (2010). Two-state Conformational Equilibrium in the Par-4 Leucine Zipper Domain. PROTEINS: Structure, Function and Bioinformatics , pp. 2433-2449.
Claridge, J. K., Heady, S. J., Chow, J., Schwalbe, M., Edwards, P. B., Jeffries, C. M., Venugopal, H., Trewhella, J. and Pascal, s. M. (2009). A picornaviral loop-to-loop replication complex. Journal of Structural Biology , pp. 251-262.
Libich, D. S., Schwalbe, M., Kate, S., Venugopal, H., Claridge, J. K., Edwards, P. B., Dutta, K. and Pascal, S. M. (2009). Intrinsic disorder and coiled coil formation in Prostate Apoptosis Response Factor 4. FEBA J. , pp. 4134-4152.
Dutta, K., Cox, C. J., Basavappa, R. and Pascal, S. (2008). 15N relaxation studies of Apo-Mts1: a dynamic S100 protein. Biochemistry 47 (29) , pp. 7637–7647.
Headey, S. J., Huang, H., Claridge, J. K., Soares, G. A., Dutta, K., Schwalbe, M., Yang, D. and Pascal, S. (2007). NMR structure of stem–loop D from human rhinovirus-14. Rna 13 (3) , pp. 351–360.
Wu, J., Fan, J., Pascal, S. and Yang, D. (2004). General method for suppression of diagonal peaks in heteronuclear-edited NOESY spectroscopy. Journal of the American Chemical Society 126 (46) , pp. 15018–15019.
Mukherjee, M., Dutta, K., Pascal, S. and Fox, R. O. (2004). Letter to the Editor: Backbone and side chain resonance assignments of domain III of the tick-borne Langat flavivirus envelope protein. Journal of biomolecular NMR 29 (4) , pp. 535–536.
Dutta, K., Engler, F. A., Cotton, L., Alexandrov, A., Bedi, G. S., Colquhoun, J. and Pascal, S. (2003). Stabilization of a pH-sensitive apoptosis-linked coiled coil through single point mutations. Protein science 12 (2) , pp. 257–265.
Dutta, K., Cox, C. J., Huang, H., Basavappa, R. and Pascal, S. (2002). Calcium coordination studies of the metastatic Mts1 protein. Biochemistry 41 (13) , pp. 4239–4245.
Dutta, K., Cox, C. J., Alexandrov, A., Huang, H., Basavappa, R. and Pascal, S. (2002). Letter to the Editor: Sequence-specific chemical shift assignment and chemical shift indexing of murine apo-Mts1. Journal of biomolecular NMR 22 (2) , pp. 181–182.
Cox, C. J., Dutta, K., Basavappa, R., Zain, S. B. and Pascal, S. (2002). Letter to the Editor: Sequence-specific chemical shift assignment of calcium-loaded murine S100A4. Journal of biomolecular NMR 23 (2) , pp. 153–154.
Cox, C. J., Dutta, K., Petri, E. T., Hwang, W. C., Lin, Y., Pascal, S. and Basavappa, R. (2002). The regions of securin and cyclin B proteins recognized by the ubiquitination machinery are natively unfolded. FEBS letters 527 (1-3) , pp. 303–308.
Alexandrov, A., Dutta, K. and Pascal, S. (2001). MBP fusion protein with a viral protease cleavage site: one-step cleavage/purification of insoluble proteins.. Biotechniques 30 (6) , pp. 1194–1198.
Dutta, K., Alexandrov, A., Huang, H. and Pascal, S. (2001). pH-induced folding of an apoptotic coiled coil. Protein Science 10 (12) , pp. 2531–2540.
Huang, H., Alexandrov, A., Chen, X., Barnes, T. W., Zhang, H., Dutta, K. and Pascal, S. (2001). Structure of an RNA hairpin from HRV-14. Biochemistry 40 (27) , pp. 8055–8064.
Arumugam, S., Pascal, S., North, C., Hu, W., Lee, K., Cotten, M., Ketchem, R., Xu, F., Brenneman, M. and Kovacs, F. (1997). Conformational Trapping in a Membrane Environment: A Regulatory Mechanism for Protein Activity. CHEMTRACTS BIOCHEMISTRY AND MOLECULAR BIOLOGY 10 , pp. 246–250.
FormanKay, J., Farrow, N., Pascal, S., Singer, A., Yamazaki, T. and Kay, L. (1996). NMR structural and dynamic studies of SH2 domain phosphopeptide complexes. Biophysical Journal 70 (2) , pp. TUAM3–TUAM3.
Yamazaki, T., Pascal, S., Singer, A. U., Formankay, J. D., Kay, L. E. and others (1995). NMR pulse schemes for the sequence-specific assignment of arginine guanidino N-15 and H-1 chemical-shifts in proteins. Journal of the American Chemical Society 117 (12) , pp. 3556–3564.
Pascal, S., Yamazaki, T., Singer, A. U., Kay, L. E. and Forman-Kay, J. D. (1995). Structural and dynamic characterization of the phosphotyrosine binding region of a Src homology 2 domain–phosphopeptide complex by NMR relaxation, proton exchange, and chemical shift approaches.. Biochemistry 34 (36) , pp. 11353–11362.
Farrow, N. A., Muhandiram, R., Singer, A. U., Pascal, S., Kay, C. M., Gish, G., Shoelson, S. E., Pawson, T., Forman-Kay, J. D. and Kay, L. E. (1994). Backbone dynamics of a free and phosphopeptide-complexed Src homology 2 domain studied by 15N NMR relaxation.. Biochemistry 33 (19) , pp. 5984–6003.
Pascal, S., Singer, A. U., Gish, G., Yamazaki, T., Shoelson, S. E., Pawson, T., Kay, L. E. and Forman-Kay, J. D. (1994). Nuclear magnetic resonance structure of an SH2 domain of phospholipase C-$γ$1 complexed with a high affinity binding peptide. Cell 77 (3) , pp. 461–472.
Pascal, S. and Cross, T. (1994). Polypeptide conformational space: dynamics by solution NMR disorder by x-ray crystallography. Journal of molecular biology 241 (3) , pp. 431–439.
Pascal, S., Muhandiram, D. Ranjith., Yamazaki, T., Formankay, J. D. and Kay, L. E. (1994). Simultaneous acquisition of 15N-and 13C-edited NOE spectra of proteins dissolved in H2O. Journal of Magnetic Resonance, Series B 103 (2) , pp. 197–201.
Pascal, S. and Cross, T. (1993). High-resolution structure and dynamic implications for a double-helical gramicidin A conformer. Journal of biomolecular NMR 3 (5) , pp. 495–513.
Zhang, Z., Pascal, S. and Cross, T. (1992). A conformational rearrangement in gramicidin A: from a double-stranded left-handed to a single-stranded right-handed helix.. Biochemistry 31 (37) , pp. 8822–8828.
Pascal, S. and Cross, T. (1992). Structure of an isolated gramicidin A double helical species by high-resolution nuclear magnetic resonance. Journal of molecular biology 226 (4) , pp. 1101–1109.

Books

Pascal, S. (2023). Understanding Quantum Science: a concise primer for students of Chemistry, Biochemistry and Physics. CRC press.
Dingley, A. J. and Pascal, S. M., editors. (2011). Biomolecular NMR Spectroscopy. Ios Press.
Pascal, S. M. (2008). NMR primer: An HSQC-based approach (with vector animations).

Book Chapters

Libich, D. S., Pandey, S. and Pascal, S. (2022). Conformational studies of the Par-4 C-terminal Domain Tumor Suppressor Par-4: Structural Features, Molecular Mechanisms and Function (pp. 95-126) Nature/Springer-Verlag.
Forman-Kay, J. D., Pascal, S., Singer, A. U., Yamazaki, T., Zhang, O., Farrow, N. A. and Kay, L. E. (1996). Structural, Dynamic, and Folding Studies of SH2 and SH3 Domains NMR as a Structural Tool for Macromolecules (pp. 35–47) Springer US.

Conference Proceeding

Arumugam, S., Pascal, S., North, C., Hu, W., Lee, K., Cotten, M., Ketchem, R., Xu, F., Brenneman, M., Kovacs, F. and others (1996). Conformational trapping in a membrane environment: a regulatory mechanism for protein activity? (pp. 5872–5876) Proceedings of the National Academy of Sciences.
FormanKay, J., Muhandiram, D., Farrow, N., Pascal, S., Yamazaki, T. and Kay, L. (1996). Dynamics in protein complexes: Relationship between motion and specific binding (pp. C9–C9) FASEB Journal .
Yamazaki, T., Pascal, S., Muhandiram, D., Lee, W., Arrowsmith, C., Farrow, N., Zhang, O., Formankay, J. and Kay, L. (1995). Pulsed-field gradient triple-resonance experiments for the study of the structure and dynamics of proteins (pp. 17–17) Journal of Cellular Biochemistry.
Pascal, S., Singer, A., Kay, L. and Formankay, J. (1995). Rrefined structure and side-chain dynamics of an SH2 domain from PLC-GAMMA-1 complexed with a PY1021 peptide from PDGFR (pp. 33–33) Journal of Cellular Biochemistry.
Cross, T., North, C., Pascal, S., Ketchum, R., Hu, W. and Lee, K. (1993). High-resolution structure, dynamics and function of the gramicidin-A channel (pp. 204–204) Journal of Cellular Biochemistry.
PASCAL, S. and CROSS, T. (1992). A RIGHT-HANDED PARALLEL DOUBLE HELICAL STRUCTURE OF GRAMICIDIN A BY HIGH-RESOLUTION NMR FASEB JOURNAL (pp. A268–A268).
TENG, Q., PASCAL, S. and CROSS, T. (1990). DETERMINATION OF TORSION ANGLES IN THE BACKBONE OF THE GRAMICIDIN CATION CHANNEL BY SOLID-STATE NMR BIOPHYSICAL JOURNAL (pp. A100–A100).

Presentations

( 2021). Conformational studies of the caspase-cleaved Par-4 (cl-Par-4) tumor suppressor protein in the presence of various ions Poster American Chemical Society National Meeting (Virtual) .
( 2021). Conformational Studies of the Caspase-Cleaved Par-4 (cl-Par-4): Influence of Monovalent & Divalent Ions on the Conformational State of the Caspase-cleaved Par-4 (cl-Par-4) Tumor Suppressor Poster Graduate Research Achievement Day (Virtual) .
( 2020). Biophysical characterization of the full-length Par-4 tumor suppressor Poster American Chemical Society National Meeting (canceled due to Covid) .
( 2020). Roles of Various Cations in the Conformational Change of Caspase-Cleaved Par-4 (cl-Par-4) Tumor Suppressor Protein Poster American Chemical Society National Meeting (canceled due to Covid) .
( 2020). Tetramerization of the caspase-activated Par-4 tumor suppressor Oral Presentation American Chemical Society National Meeting (canceled due to Covid) .
( 2019). Acid-induced folding of caspase-activated Par-4 tumor suppressor Oral Presentation American Chemical Society Spring 2019 National Meeting .
( 2019). The caspase-cleaved Par-4 tumor suppressor folds at acidic pH Poster Graduate Research Achievement Day .
( 2019). Interactions Between Anti-Cancer Drugs and the Par-4 Tumor Suppressor Poster Virginia Acacademy of Sciences .
( 2019). Ionic strength-induced tetramer formation in the Par-4 tumor suppressor Oral Presentation Virginia Academy of Science .
( 2019). Folding of the intrinsically disordered Par-4 tumor suppressor under native and extreme conditions Oral Presentation Virginia Space Grant Consortium Spring Meeting .
( 2018). Folding of the Intrinsically Disordered Par-4 Tumor Suppressor and its Role in Cisplatin Treatment of Cancer Poster Eastern Virginia Medical School Graduate Student Research Conference .
( 2018). Folding of the Intrinsically Disordered Par-4 Tumor Suppressor and its Role in Cisplatin Treatment of Cancer Poster Graduate Research Achievement Day .
( 2018). Structural Determination of the Picornavirus Replication Complex as a Potential Target for Novel Therapeutics Keynote/Plenary Address Keynote Poster Presentation, Eastern Virginia Medical School .
( 2018). Folding of the Intrinsically Disordered Par-4 Tumor Suppressor and its Role in Cisplatin Treatment of Cancer Oral Presentation Virginia Academy of Science .
( 2017). Structure of the RNA Stem Loop that Circularizes the Picornavirus Genome Oral Presentation Graduate Achievement Day .
Warden, M. S., Tonelli, M., Cornilescu, G., Liu, D., Hopersberger, L. J., Ponniah, K. and Pascal, S. M. ( 2017). Structure of an RNA stem loop that directs picornavirus replication Oral Presentation Viriginia Academy of Sciences Annual Meeting VCU, Richmond.
Warden, M. S., Tonelli, M., Cornilescu, G., Liu, D., Hopersberger, L. J., Ponniah, K. and Pascal, S. M. ( 2017). Structure of the RNA stem loop that circularizes the picornavirus genome Oral Presentation Washington Area NMR users group annual meeting Bethesda.
( 2016). Bigger is Better, but Less is More: an NMR-centered approach to large systems Oral Presentation Frank Reidy Research Center for BioElectrics seminar ODU.
( 2015). Structural Analysis and Prediction of Protein Phosphorylation Sites Poster ISBRA (International Symposium on Bioinformatics Research and Applications) Norfolk.
( 2014). Hunting Rhinos in Hampton Roads (and other viruses) Oral Presentation Invited Seminar, Virginia Commonwealth University .
( 2013). Hunting Rhinos in Hampton Roads (and other viruses) Oral Presentation Invited Seminar, Eastern Virginia Medical School .
( 2013). Hunting Rhinos in Hampton Roads (and other viruses) Oral Presentation Invited seminar, George Mason University (canceled due to snow storm) .
( 2013). Hunting Rhinos in Hampton Roads (and other viruses) Oral Presentation Invited seminar, National Cancer Institute .
  • 2020: $433,00 NIH R21 award: Synthesis of enterovirus positive-strand RNAs: discovery of viral and host determinants of RNP complex formation,
  • 2010: NZD 735,000 Royal Society Grant: Rhinoviral cloverleaf structure vis NMR/SAXS,
  • 2008: NZD 83,000 Hort Research Grant: Biomed Applications of Plant,
  • 2006: Distinguighed Teaching Award, Institute of Fundamental Sciences
  • 2006: Innovation & Excellence in Teaching Award: develop NMR visual for computer ,
  • 2006: Innovation and Excellence in Teaching award: develop NMR visual aids for computer,
  • 2006: Member, Board of Directors, ANZMAG, Australia-NZ Magnetic Resonance Society
  • 2006: NZD 720,000 Royal Society Grant: Apoptosis/Cancer/Alzheimers via NMR,
  • 2005: Distinguished Visiting Scholar NYSBC,
  • 2005: NZD 75,000 Lottery Health Grant: Auto-tune/Match Module for NMR cryoprobe,
  • 2004: NZD 750,000 Royal Society Grant: Picornaviral replication studies via NMR,
  • 2004: Taught at EMBO mirror BioNMR workshop in Australia, G. Otting
  • 1998: USD 275,000 DOD Grant Award to study Prostate Cancer Molecules via NMR,
  • 1997: Michael Kashs Lecturer - outstanding alumnus, Florida St. Molecular Biophys Program
  • 1996: Andrew Sass-Kortsak Award - Outstanding Fellow, Hospital for Sick Children
  • 1993: Doctoral Degree with Honours,
  • 1993: Medical Research Council Postdoctoral Fellowship,
  • 1991: University Fellowship,
  • 1990: University Fellowship,
  • 1989: University Fellowship,
  • 1988: College Teaching Fellowship,
  • 1988: Research Fellowship,
  • 1988: University Fellowship,
  • 1987: Research Fellowship,
  • 1985: Graduation with Highest Distinction,
  • 1985: Phi Beta Kappa,
  • 1983: Board of Governors Scholarship,
  • 1981: Board of Governors Scholarship,
  • 1981: National Merit Finalist,
  • 1981: Performance Scholarship, Music Department
  • 1981: Westinghouse Scholarship,