Electroporation Quantification and Modeling
Frank Reidy Research Center for Bioelectrics Seminar
"Quantification of electroporation via experiments and modeling: What do we learn?"
Hao Lin, PhD
Department of Mechanical and Aerospace Engineering
Rutgers, The State University of New Jersey
Tuesday, December 3, 2013, 9-10AM
IRP2 First Floor Conference Room
4211 Monarch Way
In this talk, results and thoughts on understanding the basic processes in electroporation-mediated molecular delivery will be presented, and both experimental and modeling aspects will be discussed. For the former, the delivery of dextran, a mid-size molecule was studied systematically with flow-cytometry. A two-pulse scheme was used, where a constant first pulse (HV) was followed by the second one (LV) with varied strength and duration. The results reveal that delivery rate exhibits a sigmoidal correlation with the strength of the LV. A cell viability assay with 7-ADD, on the other hand, demonstrates a convincing linear dependence on the electrical energy. For the latter, a transport model is constructed by combining an asymptotic Smoluchowski equation for membrane permeabilization with Nernst-Planck equations for ionic transport. The model predicts both pore dynamics and species concentration evolution on a single-cell level. A comparison between the experimental and modeling results leads to significant physical insights. A perspective on the development of a rational optimization approach will also be discussed.
Posted By: Loree Heller
Date: Mon Nov 25 09:51:07 EST 2013