Steven Pascal

PROFESSOR
Chemistry & Biochemistry

Contact Information

CHEMISTRY BUILDING 4501 ELKHORN AVE
NORFOLK, VA 23529

Email: spascal@odu.edu

Phone: 757-683-6763

Education/Credentials

Web Link(s)

Pascal Group

Expertise

Chemistry

NMR, Structural Biology, Structure Prediction

Biology

Viruses, Cancer, Structural Biology, Structure and Function

Physics

Biophysics,SAXS,Bioinformatics
Research Interests

Combining NMR techniques with Small Angle X-ray Scattering (SAXS) and bioinformatics to determine protein and nucleic acid structure.

Articles

Raut, K. K., Ponniah, K., and Pascal, S. M. (2021). Structural Analysis of the cl-Par-4 Tumor Suppressor as a Function of Ionic Environment. Biomolecules, 11 (386), pp. 1-13.

Bowitch, A., Sahoo, A., Clark, A. M., Ntangka, C., Raut, K. K., Gollnick, P., Yu, M. C., Pascal, S. M., Walker, S. E., and Ferkey, D. M. (2021). Methylation of the D2 dopamine receptor affects binding with the human regulatory proteins Par-4 and Calmodulin. Micropublication Biology, Caltech Library

Pascal, S., Garimella, R., Warden, M. S., and Ponniah, K. (2020). Structural biology of the enterovirus replication-linked 5'-clover leaf RNA and associated virus proteins. Microbiology and Molecular Biology Reviews, 84(2),

Wortman, M., Dagdanova, A., Clark, A., Earl, G., Pascal, S., Johnson, E., and Dianne, D. (2020). A Synthetic Pur-based Peptide Binds and Alters G-quadruplex Secondary Structure Present in the Expanded RNA Repeat of C9orf72 ALS/FTD. BBA - Molecular Cell Research, 1867,

Clark, A. M., Ponniah, K., Warden, M. S., Raitt, E. M., Smith, B. G., and Pascal, S. (2019). Tetramer formation by the caspase-activated fragment of the Par-4 tumor suppressor. FEBS Journal, 286, pp. 4060-4073.

Warden, M. S., Cai, K., Cornilescu, G., Burke, J. E., Ponniah, K., Butcher, S. E., and Pascal, S. (2019). Conformational Flexibility in the Enterovirs RNA Replication Platform. RNA, 25, pp. 376-387.

Clark, A. M., Ponniah, K., Warden, M. S., Raitt, E. M., Yawn, A. C., and Pascal, S. (2018). pH-induced Folding of the Caspase-cleaved Par-4 Tumor Suppressor: Evidence of Structure Outside of the Coiled Coil Domain. Biomolecules, 8 (4), pp. 162(17).

Abdelrasoul, M., Ponniah, K., Mao, A., Warden, M. S., Elhefnawy, W., Li, Y., and Pascal, S. (2017). Conformational Clusters of Phosphorylated Tyrosine. JACS (Journal of the American Chemical Society), 139, pp. 17632-17638.

Warden, M., Tonelli, M., Cornilescu, G., Liu, D., Hopersberger, L., Ponniah, K., and Pascal, S. (2017). Structure of RNA stem loop B from the picornavirus replication platform. Biochemistry, 56 (20), pp. 2549-2557.

Sharp, J. A., Hair, P. S., Pallera, H. K., Kumar, P. S., Mauriello, C. T., Nyalwidhe, J. O., Phelps, C. A., Park, D., Thielens, N. M., Pascal, S. M., Chen, W., Duffy, D. M., Lattanzio, F. A., Cunnion, K. M., and Krishana, N. K. (2015). Peptide Inhibitor of Complement C1 (PIC1) Rapidly Inhibits Complement Activation after Intravascular Injection in Rats. PLOS

Elhefnawy, W., Boudion, A., O’Saben, E., Abdelaal, M., Pascal, S., and Li, Y. (2015). Structural Analysis and Prediction of Protein Phosphorylation Sites. ISBRA 2015 pp. 5.

Suzuki, R., Irie, R., Harntaweesup, Y., Tachibana, K., Holland, P. T., Harwood, D. T., Shi, F., Beuzenberg, V., Itoh, Y., Pascal, S. M., Edwards, P. B., and Satake, M. (2014). Brevisulcatic Acids, Marine Ladder-Frame Polyethers from the Red Tide Dinoflagellate Karenia brevisulcata in New Zealand. Organic Letters, 16, pp. 1580-1583.

Li, Q., Pascal, S., and Li, Y. (2014). Intrinsically Disorder Protein Prediction using Undersampling Feedforward Neural Networks and Predicted Amino Acid Features. Proceedings of Modeling, Simulation, and Visualization Capstone Conference

Holland, P. T., Shi, F., Satake, M., Hamamoto, Y., Ito, E., Beuzenberg, V., McNabb, P., Munday, R., Briggs, L., Truman, P., Gooneratne, R., Edwards, P., and Pascal, S. M. (2012). Novel toxins produced by the dinoflagellate Karenia brevisulcata. Harmful Algae

Venugopal, H., Edwards, P. J., Schwalbe, M., Claridge, J. K., Libich, D. S., Stepper, J., Loo, T., Patchett, M. L., Norris, G. E., and Pascal, S. (2011). Structural, dynamic, and chemical characterization of a novel S-glycosylated bacteriocin. Biochemistry, 50 (14), pp. 2748–2755.

Sun, X., Jones, W. T., Harvey, D., Edwards, P. J., Pascal, S., Kirk, C., Considine, T., Sheerin, D. J., Rakonjac, J., Oldfield, C. J., and others (2010). N-terminal domains of DELLA proteins are intrinsically unstructured in the absence of interaction with GID1/gibberellic acid receptors. Journal of Biological Chemistry, 285 (15), pp. 11557–11571.

Edwards, P. J., Kakubayashi, M., Dykstra, R., Pascal, S., and Williams, M. A. (2010). Rheo-NMR studies of an enzymatic reaction: evidence of a shear-stable macromolecular system. Biophysical journal, 98 (9), pp. 1986–1994.

Ponniah, K., Loo, T. S., Edwards, P. J., Pascal, S., Jameson, G. B., and Norris, G. E. (2010). The production of soluble and correctly folded recombinant bovine $β$-lactoglobulin variants A and B in Escherichia coli for NMR studies. Protein expression and purification, 70 (2), pp. 283–289.

Heady, S. J., MacAskill, U. K., Wright, M. A., Claridge, J. K., Edwards, P. B., Farley, P. C., Christeller, J. T., Laing, W. A., and Pascal, S. M. (2010). The Solution structure of the squash aspartic acid proteinase inhibitor (SQAPI) and mutational analysis of pepsin inhibition. J.Biol.CHem

Schwalbe, M., Dutta, K., Libich, D. S., Venugopal, H., Claridge, J. K., Gell, D. A., Mackay, J. P., Edwards, P. B., and Pascal, S. M. (2010). Two-state Conformational Equilibrium in the Par-4 Leucine Zipper Domain. PROTEINS: Structure, Function and Bioinformatics pp. 2433-2449.

Claridge, J. K., Heady, S. J., Chow, J., Schwalbe, M., Edwards, P. B., Jeffries, C. M., Venugopal, H., Trewhella, J., and Pascal, s. M. (2009). A picornaviral loop-to-loop replication complex. Journal of Structural Biology pp. 251-262.

Libich, D. S., Schwalbe, M., Kate, S., Venugopal, H., Claridge, J. K., Edwards, P. B., Dutta, K., and Pascal, S. M. (2009). Intrinsic disorder and coiled coil formation in Prostate Apoptosis Response Factor 4. FEBA J. pp. 4134-4152.

Dutta, K., Cox, C. J., Basavappa, R., and Pascal, S. (2008). 15N relaxation studies of Apo-Mts1: a dynamic S100 protein. Biochemistry, 47 (29), pp. 7637–7647.

Headey, S. J., Huang, H., Claridge, J. K., Soares, G. A., Dutta, K., Schwalbe, M., Yang, D., and Pascal, S. (2007). NMR structure of stem–loop D from human rhinovirus-14. Rna, 13 (3), pp. 351–360.

Wu, J., Fan, J., Pascal, S., and Yang, D. (2004). General method for suppression of diagonal peaks in heteronuclear-edited NOESY spectroscopy. Journal of the American Chemical Society, 126 (46), pp. 15018–15019.

Mukherjee, M., Dutta, K., Pascal, S., and Fox, R. O. (2004). Letter to the Editor: Backbone and side chain resonance assignments of domain III of the tick-borne Langat flavivirus envelope protein. Journal of biomolecular NMR, 29 (4), pp. 535–536.

Dutta, K., Engler, F. A., Cotton, L., Alexandrov, A., Bedi, G. S., Colquhoun, J., and Pascal, S. (2003). Stabilization of a pH-sensitive apoptosis-linked coiled coil through single point mutations. Protein science, 12 (2), pp. 257–265.

Dutta, K., Cox, C. J., Huang, H., Basavappa, R., and Pascal, S. (2002). Calcium coordination studies of the metastatic Mts1 protein. Biochemistry, 41 (13), pp. 4239–4245.

Dutta, K., Cox, C. J., Alexandrov, A., Huang, H., Basavappa, R., and Pascal, S. (2002). Letter to the Editor: Sequence-specific chemical shift assignment and chemical shift indexing of murine apo-Mts1. Journal of biomolecular NMR, 22 (2), pp. 181–182.

Cox, C. J., Dutta, K., Basavappa, R., Zain, S. B., and Pascal, S. (2002). Letter to the Editor: Sequence-specific chemical shift assignment of calcium-loaded murine S100A4. Journal of biomolecular NMR, 23 (2), pp. 153–154.

Cox, C. J., Dutta, K., Petri, E. T., Hwang, W. C., Lin, Y., Pascal, S., and Basavappa, R. (2002). The regions of securin and cyclin B proteins recognized by the ubiquitination machinery are natively unfolded. FEBS letters, 527 (1-3), pp. 303–308.

Alexandrov, A., Dutta, K., and Pascal, S. (2001). MBP fusion protein with a viral protease cleavage site: one-step cleavage/purification of insoluble proteins.. Biotechniques, 30 (6), pp. 1194–1198.

Dutta, K., Alexandrov, A., Huang, H., and Pascal, S. (2001). pH-induced folding of an apoptotic coiled coil. Protein Science, 10 (12), pp. 2531–2540.

Huang, H., Alexandrov, A., Chen, X., Barnes, T. W., Zhang, H., Dutta, K., and Pascal, S. (2001). Structure of an RNA hairpin from HRV-14. Biochemistry, 40 (27), pp. 8055–8064.

Arumugam, S., Pascal, S., North, C., Hu, W., Lee, K., Cotten, M., Ketchem, R., Xu, F., Brenneman, M., and Kovacs, F. (1997). Conformational Trapping in a Membrane Environment: A Regulatory Mechanism for Protein Activity. CHEMTRACTS BIOCHEMISTRY AND MOLECULAR BIOLOGY, 10, pp. 246–250.

FormanKay, J., Farrow, N., Pascal, S., Singer, A., Yamazaki, T., and Kay, L. (1996). NMR structural and dynamic studies of SH2 domain phosphopeptide complexes. Biophysical Journal, 70 (2), pp. TUAM3–TUAM3.

Yamazaki, T., Pascal, S., Singer, A. U., Formankay, J. D., Kay, L. E., and others (1995). NMR pulse schemes for the sequence-specific assignment of arginine guanidino N-15 and H-1 chemical-shifts in proteins. Journal of the American Chemical Society, 117 (12), pp. 3556–3564.

Pascal, S., Yamazaki, T., Singer, A. U., Kay, L. E., and Forman-Kay, J. D. (1995). Structural and dynamic characterization of the phosphotyrosine binding region of a Src homology 2 domain–phosphopeptide complex by NMR relaxation, proton exchange, and chemical shift approaches.. Biochemistry, 34 (36), pp. 11353–11362.

Farrow, N. A., Muhandiram, R., Singer, A. U., Pascal, S., Kay, C. M., Gish, G., Shoelson, S. E., Pawson, T., Forman-Kay, J. D., and Kay, L. E. (1994). Backbone dynamics of a free and phosphopeptide-complexed Src homology 2 domain studied by 15N NMR relaxation.. Biochemistry, 33 (19), pp. 5984–6003.

Pascal, S., Singer, A. U., Gish, G., Yamazaki, T., Shoelson, S. E., Pawson, T., Kay, L. E., and Forman-Kay, J. D. (1994). Nuclear magnetic resonance structure of an SH2 domain of phospholipase C-$γ$1 complexed with a high affinity binding peptide. Cell, 77 (3), pp. 461–472.

Pascal, S., and Cross, T. (1994). Polypeptide conformational space: dynamics by solution NMR disorder by x-ray crystallography. Journal of molecular biology, 241 (3), pp. 431–439.

Pascal, S., Muhandiram, D. R., Yamazaki, T., Formankay, J. D., and Kay, L. E. (1994). Simultaneous acquisition of 15N-and 13C-edited NOE spectra of proteins dissolved in H2O. Journal of Magnetic Resonance, Series B, 103 (2), pp. 197–201.

Pascal, S., and Cross, T. (1993). High-resolution structure and dynamic implications for a double-helical gramicidin A conformer. Journal of biomolecular NMR, 3 (5), pp. 495–513.

Zhang, Z., Pascal, S., and Cross, T. (1992). A conformational rearrangement in gramicidin A: from a double-stranded left-handed to a single-stranded right-handed helix.. Biochemistry, 31 (37), pp. 8822–8828.

Pascal, S., and Cross, T. (1992). Structure of an isolated gramicidin A double helical species by high-resolution nuclear magnetic resonance. Journal of molecular biology, 226 (4), pp. 1101–1109.
Books

Pascal, S. A Quantum Primer for Biochemistry Students. Oxford.

Dingley, A. J., and Pascal, S. (2011). Biomolecular NMR Spectroscopy. Ios Press.
Book Chapters

Libich, D. S., Pandey, S., and Pascal, S. (2022). Conformational studies of the Par-4 C-terminal Domain.Tumor Suppressor Par-4: Structural Features, Molecular Mechanisms and Function (pp. 95-126). Springer-Verlag.

Forman-Kay, J. D., Pascal, S., Singer, A. U., Yamazaki, T., Zhang, O., Farrow, N. A., and Kay, L. E. (1996). Structural, Dynamic, and Folding Studies of SH2 and SH3 Domains.NMR as a Structural Tool for Macromolecules (pp. 35–47). Springer US.
Conference Proceeding

Arumugam, S., Pascal, S., North, C., Hu, W., Lee, K., Cotten, M., Ketchem, R., Xu, F., Brenneman, M., Kovacs, F., and others (1996). Conformational trapping in a membrane environment: a regulatory mechanism for protein activity?. (pp. 5872–5876). Proceedings of the National Academy of Sciences.

FormanKay, J., Muhandiram, D., Farrow, N., Pascal, S., Yamazaki, T., and Kay, L. (1996). Dynamics in protein complexes: Relationship between motion and specific binding. (pp. C9–C9). FASEB Journal.

Yamazaki, T., Pascal, S., Muhandiram, D., Lee, W., Arrowsmith, C., Farrow, N., Zhang, O., Formankay, J., and Kay, L. (1995). Pulsed-field gradient triple-resonance experiments for the study of the structure and dynamics of proteins. (pp. 17–17). Journal of Cellular Biochemistry.

Pascal, S., Singer, A., Kay, L., and Formankay, J. (1995). Rrefined structure and side-chain dynamics of an SH2 domain from PLC-GAMMA-1 complexed with a PY1021 peptide from PDGFR. (pp. 33–33). Journal of Cellular Biochemistry.

Cross, T., North, C., Pascal, S., Ketchum, R., Hu, W., and Lee, K. (1993). High-resolution structure, dynamics and function of the gramicidin-A channel. (pp. 204–204). Journal of Cellular Biochemistry.

PASCAL, S., and CROSS, T. (1992). A RIGHT-HANDED PARALLEL DOUBLE HELICAL STRUCTURE OF GRAMICIDIN A BY HIGH-RESOLUTION NMR. ,FASEB JOURNAL (pp. A268–A268)

TENG, Q., PASCAL, S., and CROSS, T. (1990). DETERMINATION OF TORSION ANGLES IN THE BACKBONE OF THE GRAMICIDIN CATION CHANNEL BY SOLID-STATE NMR. ,BIOPHYSICAL JOURNAL (pp. A100–A100)
Presentations

(2021). Conformational studies of the caspase-cleaved Par-4 (cl-Par-4) tumor suppressor protein in the presence of various ions Poster presented at American Chemical Society National Meeting (Virtual).

(2021). Conformational Studies of the Caspase-Cleaved Par-4 (cl-Par-4): Influence of Monovalent & Divalent Ions on the Conformational State of the Caspase-cleaved Par-4 (cl-Par-4) Tumor Suppressor Poster presented at Graduate Research Achievement Day (Virtual).

(2020). Biophysical characterization of the full-length Par-4 tumor suppressor Poster presented at American Chemical Society National Meeting (canceled due to Covid).

(2020). Roles of Various Cations in the Conformational Change of Caspase-Cleaved Par-4 (cl-Par-4) Tumor Suppressor Protein Poster presented at American Chemical Society National Meeting (canceled due to Covid).

(2020). Tetramerization of the caspase-activated Par-4 tumor suppressor Oral Presentation presented at American Chemical Society National Meeting (canceled due to Covid).

(2019). Acid-induced folding of caspase-activated Par-4 tumor suppressor Oral Presentation presented at American Chemical Society Spring 2019 National Meeting.

(2019). The caspase-cleaved Par-4 tumor suppressor folds at acidic pH Poster presented at Graduate Research Achievement Day.

(2019). Interactions Between Anti-Cancer Drugs and the Par-4 Tumor Suppressor Poster presented at Virginia Acacademy of Sciences.

(2019). Ionic strength-induced tetramer formation in the Par-4 tumor suppressor Oral Presentation presented at Virginia Academy of Science.

(2019). Folding of the intrinsically disordered Par-4 tumor suppressor under native and extreme conditions Oral Presentation presented at Virginia Space Grant Consortium Spring Meeting.

(2018). Folding of the Intrinsically Disordered Par-4 Tumor Suppressor and its Role in Cisplatin Treatment of Cancer Poster presented at Eastern Virginia Medical School Graduate Student Research Conference.

(2018). Folding of the Intrinsically Disordered Par-4 Tumor Suppressor and its Role in Cisplatin Treatment of Cancer Poster presented at Graduate Research Achievement Day.

(2018). Structural Determination of the Picornavirus Replication Complex as a Potential Target for Novel Therapeutics Keynote/Plenary Address presented at Keynote Poster Presentation, Eastern Virginia Medical School.

(2018). Folding of the Intrinsically Disordered Par-4 Tumor Suppressor and its Role in Cisplatin Treatment of Cancer Oral Presentation presented at Virginia Academy of Science.

(2017). Structure of the RNA Stem Loop that Circularizes the Picornavirus Genome Oral Presentation presented at Graduate Achievement Day.

Warden, M. S., Tonelli, M., Cornilescu, G., Liu, D., Hopersberger, L. J., Ponniah, K., and Pascal, S. M. (2017). Structure of an RNA stem loop that directs picornavirus replication Oral Presentation presented at Viriginia Academy of Sciences Annual Meeting VCU, Richmond.

Warden, M. S., Tonelli, M., Cornilescu, G., Liu, D., Hopersberger, L. J., Ponniah, K., and Pascal, S. M. (2017). Structure of the RNA stem loop that circularizes the picornavirus genome Oral Presentation presented at Washington Area NMR users group annual meeting Bethesda.

(2016). Bigger is Better, but Less is More: an NMR-centered approach to large systems Oral Presentation presented at Frank Reidy Research Center for BioElectrics seminar ODU.

(2015). Structural Analysis and Prediction of Protein Phosphorylation Sites Poster presented at ISBRA (International Symposium on Bioinformatics Research and Applications) Norfolk.

(2014). Hunting Rhinos in Hampton Roads (and other viruses) Oral Presentation presented at Invited Seminar, Virginia Commonwealth University.

(2013). Hunting Rhinos in Hampton Roads (and other viruses) Oral Presentation presented at Invited Seminar, Eastern Virginia Medical School.

(2013). Hunting Rhinos in Hampton Roads (and other viruses) Oral Presentation presented at Invited seminar, George Mason University (canceled due to snow storm).

(2013). Hunting Rhinos in Hampton Roads (and other viruses) Oral Presentation presented at Invited seminar, National Cancer Institute.

2020: $433,00 NIH R21 award: Synthesis of enterovirus positive-strand RNAs: discovery of viral and host determinants of RNP complex formation
2010: NZD 735,000 Royal Society Grant: Rhinoviral cloverleaf structure vis NMR/SAXS
2008: NZD 83,000 Hort Research Grant: Biomed Applications of Plant
2006: Distinguighed Teaching Award, Institute of Fundamental Sciences
2006: Innovation & Excellence in Teaching Award: develop NMR visual for computer
2006: Innovation and Excellence in Teaching award: develop NMR visual aids for computer
2006: Member, Board of Directors, ANZMAG, Australia-NZ Magnetic Resonance Society
2006: NZD 720,000 Royal Society Grant: Apoptosis/Cancer/Alzheimers via NMR
2005: Distinguished Visiting Scholar NYSBC
2005: NZD 75,000 Lottery Health Grant: Auto-tune/Match Module for NMR cryoprobe
2004: NZD 75,000 Royal Society Grant: Picornaviral replication studies via NMR
2004: Taught at EMBO mirror BioNMR workshop in Australia, G. Otting
1998: USD 275,000 DOD Grant Award to study Prostate Cancer Molecules via NMR
1997: Michael Kashs Lecturer - outstanding alumnus, Florida St. Molecular Biophys Program
1996: Andrew Sass-Kortsak Award - Outstanding Fellow, Hospital for Sick Children
1993: Doctoral Degree with Honours
1993: Medical Research Council Postdoctoral Fellowship
1991: University Fellowship
1990: University Fellowship
1989: University Fellowship
1988: College Teaching Fellowship
1988: Research Fellowship
1988: University Fellowship
1987: Research Fellowship
1985: Graduation with Highest Distinction
1985: Phi Beta Kappa
1983: Board of Governors Scholarship
1981: Board of Governors Scholarship
1981: National Merit Finalist
1981: Performance Scholarship, Music Department
1981: Westinghouse Scholarship

Contracts, Grants and Sponsored Research

Semler, B.; Pascal, S. "Synthesis of enterovirus positive-strand RNAs: discovery of viral and host determinants of RNP complex formation" $433,169. Federal. February, 2020 - January, 2023

, "Biophysical characterization of full-length Par-4 tumor suppressor" $1,500. Old Dominion University. January, 2019 - May, 2019

, "Par-4 tumor suppressor: structural changes that trigger the death of cancer cells" $10,000. Old Dominion University. January, 2018 - May, 2019

, "Expression and Purification of HRV-14 proteins 3D & 3CD for in vitro studies" $1,500. Old Dominion University. January, 2014 - May, 2014

, "Picornavirus RNA Template Synthesis, In Vitro Transcription and initial structural characterization" $1,500. Old Dominion University. January, 2013 - May, 2013

, "Looking over a four-leaf clover: structural characterisation of a critical picornaviral RNA clover leaf and its interaction with a viral protein" $735,000. Foreign. January, 2009 - January, 2012

, "The zipper, the butterfly and death: structural studies of an apoptosis-triggering protein-protein interaction linked to prostate cancer and neurodegenerative disease" $720,000. Federal. January, 2006 - January, 2010

, "Biomedical Applications of Plant Genes" $83,000. January, 2008 - January, 2009

, "Picornaviral Replication: Structures and Interactions of Two Key Viral Molecules" $630,000. Federal. January, 2004 - January, 2007

, "Automated Tune and Match (ATM) Module for 700 MHz NMR Cryoprobe" $75,000. Federal. January, 2005 - December, 2005

, "Structure and Interactions of a Protein Linked to Apoptosis Response in Prostate Tumors" $263,509. Federal. January, 1998 - January, 2001

Old Dominion University

Idea Fusion

Steven Pascal

Steven Pascal

Contact Information

Organizational Chart

Education/Credentials

Web Link(s)

Expertise

Research Interests

Articles

Books

Book Chapters

Conference Proceeding

Presentations

Contracts, Grants and Sponsored Research