RESEARCH PROFESSOR
Frank Reidy Research Center for Bioelectrics
Andrei Pakhomov
SUITE 300 4211 MONARCH WAY
NORFOLK, 23508
Ph.D. in Biophysics/Radiation Biology, Medical Radiology Research Center, (1989)
M.Sc. in Biology/Animal and Human Physiology, Moscow State University, (1982)
Contracts, Grants and Sponsored Research
- "Nanoelectropulse-induced electromechanical signaling and control of biological systems" $7,500,000. Federal. October 01, 2015 - September 30, 2020
- "Low Energy Defibrillation with Nanosecond Pulsed Electric Field" $1,509,283. Federal. August 12, 2015 - May 31, 2019
- Pakhomov, A., Pakhomova, O. and Semenov, I. "Nanoelectropulse-Induced Electromechanical Signaling And Control of Biological Systems" $132,941. Federal. September 30, 2015 - September 30, 2018
- Pakhomov, A., Pakhomova, O. and Semenov, I. "Nanoelectropulse-Induced Electromechanical Signaling And Control of Biological Systems" $136,579. Federal. September 30, 2015 - September 30, 2018
- Pakhomov, A., Pakhomova, O. and Semenov, I. "Nanoelectropulse-Induced Electromechanical Signaling And Control of Biological Systems" $142,937. Federal. September 30, 2015 - September 30, 2018
- Pakhomov, A., Pakhomova, O. and Semenov, I. "Nanoelectropulse-Induced Electromechanical Signaling And Control of Biological Systems" $611,260. Federal. September 30, 2015 - September 30, 2018
- Pakhomov, A., Pakhomova, O., Semenov, I., Hill, E. L., Lobova, T. and Morris, J. C. "Low Energy Defibrillation With Nanosecond Pulsed Electric Field" $151,075. Federal. June 1, 2016 - May 31, 2017
- Xiao, S. "Picosecond pulse technology for non-invasive electrostimulation" $393,393. Federal. March 1, 2014 - February 28, 2017
- Pakhomov, A., Pakhomova, O. and Xiao, S. "Mechanisms and Implications of Nanoelectroporation in living cells" $1,200,000. Federal. July 10, 2010 - June 30, 2015
- Zemlin, C. "Defibrillation of Cardiac Tissue with Nanosecond Pulsed Electric Fields" $50,000. Old Dominion University. 2013 - 2014
- Ibey, B. and Pakhomov, A. "Investigation of field-dependent bioeffects of high amplitude electromagnetic pulses" $1,730,000. Federal. 2011 - 2013
- Pakhomov, A. and Ibey, B. "Mechanism of interaction between pulsed electric field and phospholipid bilayer membrane" $623,000. Federal. 2009 - 2013
- Pakhomova, O. and Xiao, S. "Cell Death Induction by High-Voltage, Nanosecond-Duration Electric Pulses" $1,078,970. Federal. 2008 - 2013
- Pakhomov, A., Beebe, S. J., Swanson, R. J., Xiao, S. and Schoenbach, K. H. "A Comprehensive Study of Neuromuscular Effects of Nanosecond Electrical Pulses" $1,967,101. Federal. 2007 - 2010
Research Interests
ElectrophysiologyElectroporationMembrane biologyCell biology
Articles
- Semenov, I., Grigoryev, S., Neuber, J. U., Zemlin, C., Pakhomova, O., Casciola, M. and Pakhomov, A. (2018). Excitation and injury of adult ventricular cardiomyocytes by nano- to millisecond electric shocks. Scientific Reports 8 , pp. 12.
- Pakhomov, A., Grigoryev, S., Semenov, I., Casciola, M., Jiang, C. and Xiao, S. (2018). The second phase of bipolar, nanosecond-range electric pulses determines the electroporation efficiency. Bioelectrochemistry 122 , pp. 123-133.
- Gianulis, E. C., Casciola, M., Xiao, S., Pakhomova, O. and Pakhomov, A. (2018). Electropermeabilization by uni- or bipolar nanosecond electric pulses: The impact of extracellular conductivity.. Bioelectrochemistry (Amsterdam, Netherlands) 119 , pp. 10-19.
- Muratori, C., Pakhomov, A., Gianulis, E., Meads, J., Casciola, M., Mollica, P. A. and Pakhomova, O. (2017). Activation of the phospholipid scramblase tmem16f by nanosecond pulsed electric field (nspef) facilitates its diverse cytophysiological effects. Journal of Biological Chemistry.
- Varghese, F., Neuber, J. U., Xie, F., Philpott, J. M., Pakhomov, A. and Zemlin, C. (2017). Low-energy defibrillation with nanosecond electric shocks.. Cardiovascular research 113 (14) , pp. 1789-1797.
- Muratori, C., Pakhomov, A., Gianulis, E., Meads, J., Casciola, M., Mollica, P. A. and Pakhomova, O. (2017). Activation of the phospholipid scramblase TMEM16F by nanosecond pulsed electric fields (nsPEF) facilitates its diverse cytophysiological effects.. The Journal of biological chemistry 292 (47) , pp. 19381-19391.
- Jensen, S. D., Khorokhorina, V. A., Muratori, C., Pakhomov, A. and Pakhomova, O. (2017). Delayed hypersensitivity to nanosecond pulsed electric field in electroporated cells.. Scientific reports 7 (1) , pp. 10992.
- Casciola, M., Xiao, S. and Pakhomov, A. (2017). Damage-free peripheral nerve stimulation by 12-ns pulsed electric field.. Scientific reports 7 (1) , pp. 10453.
- Xiao, S., Semenov, I., Petrella, R., Pakhomov, A. and Schoenbach, K. H. (2017). A subnanosecond electric pulse exposure system for biological cells.. Medical & biological engineering & computing 55 (7) , pp. 1063-1072.
- Merla, C., Pakhomov, A., Semenov, I. and Vernier, T. (2017). Frequency spectrum of induced transmembrane potential and permeabilization efficacy of bipolar electric pulses.. Biochimica et biophysica acta 1859 (7) , pp. 1282-1290.
- Pakhomov, A., Semenov, I., Casciola, M. and Xiao, S. (2017). Neuronal excitation and permeabilization by 200-ns pulsed electric field: An optical membrane potential study with FluoVolt dye.. Biochimica et biophysica acta 1859 (7) , pp. 1273-1281.
- Gianulis, E. C., Labib, C., Saulis, G., Novickij, V., Pakhomova, O. and Pakhomov, A. (2017). Selective susceptibility to nanosecond pulsed electric field (nsPEF) across different human cell types.. Cellular and molecular life sciences : CMLS 74 (9) , pp. 1741-1754.
- Muratori, C., Pakhomov, A. and Pakhomova, O. (2017). Effect of Cooling On Cell Volume and Viability After Nanoelectroporation.. The Journal of membrane biology 250 (2) , pp. 217-224.
- Muratori, C., Pakhomov, A., Heller, L., Casciola, M., Gianulis, E., Grigoryev, S., Xiao, S. and Pakhomova, O. (2017). Electrosensitization Increases Antitumor Effectiveness of Nanosecond Pulsed Electric Fields In Vivo.. Technology in cancer research & treatment , pp. 1533034617712397.
- Muratori, C., Pakhomov, A., Gianulis, E. C., Jensen, S. D. and Pakhomova, O. (2016). The cytotoxic synergy of nanosecond electric pulses and low temperature leads to apoptosis.. Scientific reports 6 , pp. 36835.
- Dermol, J., Pakhomova, O., Pakhomov, A. and Miklavčič, D. (2016). Cell Electrosensitization Exists Only in Certain Electroporation Buffers.. PloS one 11 (7) , pp. e0159434.
- Semenov, I., Xiao, S. and Pakhomov, A. (2016). Electroporation by subnanosecond pulses.. Biochemistry and biophysics reports 6 , pp. 253-259.
- Muratori, C., Pakhomov, A., Xiao, S. and Pakhomova, O. (2016). Electrosensitization assists cell ablation by nanosecond pulsed electric field in 3D cultures.. Scientific reports 6 , pp. 23225.
- Xie, F., Varghese, F., Pakhomov, A., Semenov, I., Xiao, S., Philpott, J. and Zemlin, C. (2015). Ablation of Myocardial Tissue With Nanosecond Pulsed Electric Fields.. PloS one 10 (12) , pp. e0144833.
- Semenov, I., Xiao, S., Kang, D., Schoenbach, K. H. and Pakhomov, A. (2015). Cell stimulation and calcium mobilization by picosecond electric pulses.. Bioelectrochemistry (Amsterdam, Netherlands) 105 , pp. 65-71.
- Semenov, I., Zemlin, C., Pakhomova, O., Xiao, S. and Pakhomov, A. (2015). Diffuse, non-polar electropermeabilization and reduced propidium uptake distinguish the effect of nanosecond electric pulses.. Biochimica et biophysica acta 1848 (10 Pt A) , pp. 2118-25.
- Vernier, T., Levine, Z. A., Ho, M. C., Xiao, S., Semenov, I. and Pakhomov, A. (2015). Picosecond and Terahertz Perturbation of Interfacial Water and Electropermeabilization of Biological Membranes.. The Journal of membrane biology 248 (5) , pp. 837-47.
- Gianulis, E. C., Lee, J., Jiang, C., Xiao, S., Ibey, B. L. and Pakhomov, A. (2015). Electroporation of mammalian cells by nanosecond electric field oscillations and its inhibition by the electric field reversal.. Scientific reports 5 , pp. 13818.
- Schoenbach, K. H., Pakhomov, A., Semenov, I., Xiao, S., Pakhomova, O. and Ibey, B. L. (2015). Ion transport into cells exposed to monopolar and bipolar nanosecond pulses.. Bioelectrochemistry (Amsterdam, Netherlands) 103 , pp. 44-51.
- Pakhomov, A., Gianulis, E., Vernier, T., Semenov, I., Xiao, S. and Pakhomova, O. (2015). Multiple nanosecond electric pulses increase the number but not the size of long-lived nanopores in the cell membrane.. Biochimica et biophysica acta 1848 (4) , pp. 958-66.
- Gianulis, E. C. and Pakhomov, A. (2015). Gadolinium modifies the cell membrane to inhibit permeabilization by nanosecond electric pulses.. Archives of biochemistry and biophysics 570 , pp. 1-7.
- Pakhomov, A., Xiao, S., Pakhomova, O., Semenov, I., Kuipers, M. A. and Ibey, B. L. (2014). Disassembly of actin structures by nanosecond pulsed electric field is a downstream effect of cell swelling.. Bioelectrochemistry (Amsterdam, Netherlands) 100 , pp. 88-95.
- Pakhomov, A., Semenov, I., Xiao, S., Pakhomova, O., Gregory, B., Schoenbach, K. H., Ullery, J. C., Beier, H. T., Rajulapati, S. R. and Ibey, B. L. (2014). Cancellation of cellular responses to nanoelectroporation by reversing the stimulus polarity.. Cellular and molecular life sciences : CMLS 71 (22) , pp. 4431-41.
- Pakhomova, O., Gregory, B., Semenov, I. and Pakhomov, A. (2014). Calcium-mediated pore expansion and cell death following nanoelectroporation.. Biochimica et biophysica acta 1838 (10) , pp. 2547-54.
- Rassokhin, M. and Pakhomov, A. (2014). Cellular regulation of extension and retraction of pseudopod-like blebs produced by nanosecond pulsed electric field (nsPEF).. Cell biochemistry and biophysics 69 (3) , pp. 555-66.
- Ibey, B. L., Ullery, J. C., Pakhomova, O., Roth, C. C., Semenov, I., Beier, H. T., Tarango, M., Xiao, S., Schoenbach, K. H. and Pakhomov, A. (2014). Bipolar nanosecond electric pulses are less efficient at electropermeabilization and killing cells than monopolar pulses.. Biochemical and biophysical research communications 443 (2) , pp. 568-73.
- Pakhomova, O., Gregory, B. W.. and Pakhomov, A. (2013). Facilitation of electroporative drug uptake and cell killing by electrosensitization. J Cell Mol Med 17 (1) , pp. 154-9.
- Semenov, I., Xiao, S. and Pakhomov, A. (2013). Primary pathways of intracellular Ca(2+) mobilization by nanosecond pulsed electric field. Biochim Biophys Acta 3 , pp. 981-9.
- Semenov, I., Xiao, S., Pakhomova, O. and Pakhomov, A. (2013). Recruitment of the intracellular Ca by ultrashort electric stimuli: The impact of pulse duration. Cell Calcium 15 (13) , pp. 00083-3.
- Pakhomova , O., Gregory, B., Semenov, I. and Pakhomov, A. (2013). Two modes of cell death caused by exposure to nanosecond pulsed electric field. PLOS One 8 (7) , pp. e70278 .
- Nesin, V., Bowman, A. M.., Xiao, S. and Pakhomov, A. (2012). Cell permeabilization and inhibition of voltage-gated Ca(2+) and Na(+) channel currents by nanosecond pulsed electric field. Bioelectromagnetics 33 (5) , pp. 394-404.
- Rassokhin, M. and Pakhomov, A. (2012). Electric field exposure triggers and guides formation of pseudopod-like blebs in U937 monocytes. J Membr Biol 245 (9) , pp. 521-9.
- Nesin, V. and Pakhomov, A. (2012). Inhibition of voltage-gated Na(+) current by nanosecond pulsed electric field (nsPEF) is not mediated by Na(+) influx or Ca(2+) signaling. Bioelectromagnetics 33 (6) , pp. 443-51.
- Pakhomova, O., Khorokhorina, V. A.., Bowman, A. M.., Rodaite-Riseviciene, R., Saulis, G., Xiao, S. and Pakhomov, A. (2012). Oxidative effects of nanosecond pulsed electric field exposure in cells and cell-free media. Arch Biochem Biophys 527 (1) , pp. 55-64.
- Nesin, V. and Pakhomov, A. (2012). Inhibition of voltage-gated Na+ current by nanosecond pulsed electric field (nsPEF) is not mediated by Na+ influx or Ca2+ signaling.. Bioelectromagnetics 33 (6) , pp. 443-51.
- Ibey, B. L.., Roth, C. C.., Pakhomov, A., Bernhard, J. A.., Wilmink, G. J.. and Pakhomova, O. (2011). Dose-dependent thresholds of 10-ns electric pulse induced plasma membrane disruption and cytotoxicity in multiple cell lines. PLoS One 6 (1) , pp. 0015642.
- Pakhomova, O., Gregory, B. W.., Khorokhorina, V. A.., Bowman, A. M.., Xiao, S. and Pakhomov, A. (2011). Electroporation-induced electrosensitization. PLoS One 6 (2) , pp. 0017100.
- Nesin, O. M.., Pakhomova, O., Xiao, S. and Pakhomov, A. (2011). Manipulation of cell volume and membrane pore comparison following single cell permeabilization with 60- and 600-ns electric pulses. Biochim Biophys Acta 3 , pp. 792-801.
- Bowman, A. M.., Nesin, O. M.., Pakhomova, O. and Pakhomov, A. (2010). Analysis of plasma membrane integrity by fluorescent detection of Tl(+) uptake. J Membr Biol 236 (1) , pp. 15-26.
- Andre, F. M.., Rassokhin, M., Bowman, A. M.. and Pakhomov, A. (2010). Gadolinium blocks membrane permeabilization induced by nanosecond electric pulses and reduces cell death. Bioelectrochemistry 79 (1) , pp. 95-100.
- Ibey, B. L.., Mixon, D. G.., Payne, J. A.., Bowman, A., Sickendick, K., Wilmink, G. J.., Roach, W. P.. and Pakhomov, A. (2010). Plasma membrane permeabilization by trains of ultrashort electric pulses. Bioelectrochemistry 79 (1) , pp. 114-21.
- Ibey, B. L.., Pakhomov, A., Gregory, B. W.., Khorokhorina, V. A.., Roth, C. C.., Rassokhin, M., Bernhard, J. A.., Wilmink, G. J.. and Pakhomova, O. (2010). Selective cytotoxicity of intense nanosecond-duration electric pulses in mammalian cells. Biochim Biophys Acta 1800 (11) , pp. 1210-9.
- Nuccitelli, R., Chen, X., Pakhomov, A., Baldwin, W. H.., Sheikh, S., Pomicter, J. L.., Ren, W., Osgood, C. J., Swanson, R. J., Kolb, J. F., Beebe, S. J. and Schoenbach, K. H. (2009). A new pulsed electric field therapy for melanoma disrupts the tumor’s blood supply and causes complete remission without recurrence. Int J Cancer 125 (2) , pp. 438-45.
- Pakhomov, A., Bowman, A. M.., Ibey, B. L.., Andre, F. M.., Pakhomova, O. and Schoenbach, K. H. (2009). Lipid nanopores can form a stable, ion channel-like conduction pathway in cell membrane. Biochem Biophys Res Commun 385 (2) , pp. 181-6.
- Ibey, B. L., Xiao, S., Schoenbach, K. H., Murphy, M. R. and Pakhomov, A. (2009). Plasma Membrane Permeabilization by 60 and 600 ns Electric Pulses is Determined by the Absorbed Dose. Bioelectromagnetics 30 (2) , pp. 90-92.
- Joshi, R., Mishra, A., Song, J., Pakhomov, A. and Schoenbach, K. H. (2008). Simulation studies of ultrashort, high-intensity electric pulse induced action potential block in whole-animal nerves. IEEE Trans Biomed Eng 55 (4) , pp. 1391-8.
- Osgood, C. J., Schoenbach, K. H., Hargrave, B., Joshi, R., Kolb, J. F., Nuccitelli, R., Pakhomov, A., Stacey, M. W., Swanson, R. J., White, J., Xiao, S. and Zhang, J. (2007). Bioelectric effects of intense nanosecond pulses. IEEE Transact Dielectrics Electrical Insul 14 , pp. 1088-1109.
- Schoenbach, K. H., Hargrave, B., Joshi, R., Kolb, J. F., Osgood, C. J., Nuccitelli, R., Pakhomov, A., Swanson, R. J., Stacey, M., White, J. A., Xiao, S., Zhang, J., Beebe, S. J., Blackmore, P. F. and Buescher, E. S. (2007). Bioelectric Effects of Nanosecond Pulses. IEEE Trans. Diel. Electr. Insul. 14 (5) , pp. 1088-1109.
- Pakhomov, A., Kolb, J. F., White, J. A., Joshi, R., Xiao, S. and Schoenbach, K. H. (2007). Long-Lasting Plasma Membrane Permeabilization in Mammalian Cells by Nanosecond Pulsed Electric Field (nsPEF). Bioelectromagnetics 28 , pp. 655.
- Pakhomov, A., Shevin, R., White, J. A.., Kolb, J. F., Pakhomova, O., Joshi, R. and Schoenbach, K. H. (2007). Membrane permeabilization and cell damage by ultrashort electric field shocks. Arch Biochem Biophys 465 (1) , pp. 109-18.
- Pakhomov, A., Semenov, I., Brenner, R. and Toney, G. M.. (2006). Hydraulically coupled microejection technique for precise local solution delivery in tissues. J Neurosci Methods 155 (2) , pp. 231-40.
- Walker, 3rd, K., Pakhomova, O., Kolb, J. F., Schoenbach, K. H., Stuck, B. E.., Murphy, M. R.. and Pakhomov, A. (2006). Oxygen enhances lethal effect of high-intensity, ultrashort electrical pulses. Bioelectromagnetics 27 (3) , pp. 221-5.
- Pakhomov, A., Doyle, J., Stuck, B. E.. and Murphy, M. R.. (2003). Effects of high power microwave pulses on synaptic transmission and long term potentiation in hippocampus. Bioelectromagnetics 24 (3) , pp. 174-81.
- Pakhomov, A., Gajsek, P., Allen, L., Stuck, B. E.. and Murphy, M. R.. (2002). Comparison of dose dependences for bioeffects of continuous-wave and high-peak power microwave emissions using gel-suspended cell cultures. Bioelectromagnetics 23 (2) , pp. 158-67.
- Gajsek, P., Pakhomov, A. and Klauenberg, B. J.. (2002). Electromagnetic field standards in Central and Eastern European countries: current state and stipulations for international harmonization. Health Phys 82 (4) , pp. 473-83.
- Pakhomov, A., Mathur, S. P.., Doyle, J., Stuck, B. E.., Kiel, J. L.. and Murphy, M. R.. (2000). Comparative effects of extremely high power microwave pulses and a brief CW irradiation on pacemaker function in isolated frog heart slices. Bioelectromagnetics 21 (4) , pp. 245-54.
- Pakhomov, A., Akyel, Y., Pakhomova, O., Stuck, B. E.. and Murphy, M. R.. (1998). Current state and implications of research on biological effects of millimeter waves: a review of the literature. Bioelectromagnetics 19 (7) , pp. 393-413.
- Pakhomov, A., Prol, H. K.., Mathur, S. P.., Akyel, Y. and Campbell, C. (1997). Search for frequency-specific effects of millimeter-wave radiation on isolated nerve function. Bioelectromagnetics 18 (4) , pp. 324-34.
- Pakhomov, A., Dubovick, B. V.., Degtyariov, I. G.. and Pronkevich, A. N.. (1995). Microwave influence on the isolated heart function: I. Effect of modulation. Bioelectromagnetics 16 (4) , pp. 241-9.
- Pakhomov, A., Dubovick, B. V.., Degtyariov, I. G.. and Pronkevich, A. N.. (1995). Microwave influence on the isolated heart function: II. Combined effect of radiation and some drugs. Bioelectromagnetics 16 (4) , pp. 250-4.
Book Chapters
- Pakhomov, A. and Pakhomova, O. (2010). Nanopores: A distinct transmembrane passageway in electroporated cells Advanced Electroporation Techniques in Biology in Medicine (pp. 178–194) Boca Raton: CRC Press.