Michael Stacey

Ph.D. in Cancer Biology, University of Birmingham, (1989)

Expertise

Research Interests

Research interests.For more detail, see the Mike Stacey's Homepage tab.A basis tenant of biology is that normal differentiated cells will respond to their environments. Chondrocytes are no different, and the biomechanical and bioelectrochemical fluxes they experience result in the production of an extra cellular matrix able to withstand variable and extreme mechanical loads. To confound this, cartilage does not have a blood supply and cells experience, in fact require, hypoxic and acidic conditions to fully function. These factors all play a role in the poor ability of cartilage to regenerate. The regenerative ability of cartilage to restore itself is of great interest in sports related injuries.The biological/biomechanical/bioelectrochemical aspects of cartilage and how these interact in disease and injury reveals intriguing and multidisciplinary avenues of research. The membrane is where the cell interacts with the environment and specialized structures link the extra cellular matrix to the cells cytoskeleton and nucleus influencing gene expression. Our investigations examine the growth of chondrocytes in 3D under hypoxic conditions, changes in gene expression of membrane associated proteins, particularly those responsible for ion movement across membranes, the ion and hemi channels.The bioelectrochemistry of chondrocytes is of huge importance and is the focus of our research through a grant from the NIAMS. A microfluidic approach enables dielectric measurements of cells to be made, with correlation to ion channel and matrix gene expression. The measured impedance is modeled using a combination of physical models, such as Cole-Cole, Constant Phase Angle, Maxwell Wagner mixture, double shell models. Subcellular dielectric parameters, such as conductance and capacitance of the cell membrane and nuclear envelope, and conductivity of the cytoplasm and nucleoplasm, are obtained as a result of dielectric modeling.

Articles

Sabuncu, A. C., Stacey, M. W., Craviso, G. L., Semenova, N., Vernier, T., Leblanc, N., Chatterjee, I. and Zaklit, J. (2018). Dielectric properties of isolated adrenal chromaffin cells determined by microfluidic impedance spectroscopy. Bioelectrochemistry 119 , pp. 84-91.

Shen, J., Stacey, M. W. and Hao, Z. (2018). A Distributed-Deflection Sensor With a Built-In Probe for Conformal Mechanical Measurements of Costal Cartilage at Its Exterior Surface. IEEE Sensors Journal 18 (2) , pp. 822-829.

Dutta, D., Palmer, X. L., Kim, J., Qian, S. and Stacey, M. W. (2017). Energy dissipation mapping of cancer cells. Micron 105 , pp. 24-29.

Dutta, D., Palmer, X., Asmar, A., Stacey, M. W. and Qian, S. (2017). Nanosecond pulsed electric field induced changes in cell surface charge density. Micron 100 , pp. 45 - 49.

Asmar, A., Werner, A., Kelly, Jr., R. E., Fecteau, A. and Stacey, M. W. (2015). Presence and Localization of Pro- and Mature Forms of Biglycan and Decorin in Human Costal Cartilage Derived from Chest Wall Deformities. Austin J Musculoskelet Disord. 2 (1) , pp. 1012.

Stacey, M. W., Sabuncu, A. C.. and Beskok, A. (2014). Dielectric Characterization of Costal Cartilage Chondrocytes. Biochemical Biophysics Acta. -General Subjects 1840 (1) , pp. 146-152.

Stacey, M. W., Dutta, D., Cao, W., Asmar, A., Elsayed-Ali, H., Kelly, Jr., R. and Beskok, A. (2013). Atomic force microscopy characterization of collagen ’nanostraws’ in human costal cartilage. Micron 44 , pp. 483-7.

Sabuncu, A. C.., Stacey, M. W. and Beskok, A. (2013). Microfluidic Dielectric Spectroscopy of Costal Cartilage Chondrocytes. Biophysical Journal 104 (2) , pp. 674a-674a.

Stacey, M. W., Grubbs, J., Asmar, A., Pryor, J., Elsayed-Ali, H., Cao, W., Beskok, A., Dutta, D., Darby, D. A., Fecteau, A., Werner, A. and Kelly, Jr., R. E.. (2012). Decorin expression, straw-like structure, and differentiation of human costal cartilage. Connect Tissue Res 53 (5) , pp. 415-21.

Basu, G., Kalluri, B. S.., Sabuncu, A. C.., Osgood, C. J. and Stacey, M. W. (2012). Enhanced Killing Effect of Nanosecond Pulse Electric Fields on PANC1 and Jurkat Cell Lines in the Presence of Tween 80. Journal of Membrane Biology 245 (10) , pp. 611-616.

Horth, L., Stacey, M., Benjamin, T., Segna, K., Proud, V. K.., Nuss, D. and Kelly, Jr., R. E.. (2012). Genetic analysis of inheritance of Pectus Excavatum. J. Pediatric Genet. 1 (3) , pp. 161-173.

Sabuncu, A. C.., Grubbs, J., Qian, S., Abdel-Fattah, T. M.., Stacey, M. W. and Beskok, A. (2012). Probing nanoparticle interactions in cell culture media. Colloids Surf B Biointerfaces 95 , pp. 96-102.

Stacey, M. W., Osgood, C. J., Kalluri, B. S.., Cao, W., Elsayed-Ali, H. and Abdel-Fattah, T. (2011). Nanosecond pulse electrical fields used in conjunction with multi-wall carbon nanotubes as a potential tumor treatment. Biomed Mater 6 (1) , pp. 011002.

Stacey, M. W. and Osgood, C. J. (2011). Nanosecond pulse electrical fields used in conjunction with mutli-wall carbon nanotubes as a potential tumor treatment.. Biomed Mater 6 , pp. 011002.

Stacey, M. W., Fox, P., Buescher, S. and Kolb, J. F. (2011). Nanosecond pulsed electric field induced cytoskeleton, nuclear membrane and telomere damage adversely impact cell survival. Bioelectrochemistry 82 (2) , pp. 131-4.

Sabuncu, A. C.., Kalluri, B. S.., Qian, S., Stacey, M. W. and Beskok, A. (2010). Dispersion state and toxicity of mwCNTs in cell culture medium with different T80 concentrations. Colloids and Surfaces B-Biointerfaces 78 (1) , pp. 36-43.

Stacey, M. W., Neumann, S. A.., Dooley, A., Segna, K., Kelly, R. E.., Nuss, D., Kuhn, A. M.., Goretsky, M. J.., Fecteau, A. H.., Pastor, A. and Proud, V. K.. (2010). Variable number of tandem repeat polymorphisms (VNTRs) in the ACAN gene associated with pectus excavatum. Clinical Genetics 78 (5) , pp. 502-4.

Kolb, J. F., Mohamed, A. A. H.., Price, R. O.., Swanson, R. J., Bowman, A., Chiavarini, R. L.., Stacey, M. W. and Schoenbach, K. H. (2008). Cold atmospheric pressure air plasma jet for medical applications. Applied Physics Letters 92 (24).

Osgood, C. J., Schoenbach, K. H., Hargrave, B., Joshi, R., Kolb, J. F., Nuccitelli, R., Pakhomov, A., Stacey, M. W., Swanson, R. J., White, J., Xiao, S. and Zhang, J. (2007). Bioelectric effects of intense nanosecond pulses. IEEE Transact Dielectrics Electrical Insul 14 , pp. 1088-1109.

Sun, Y., Xiao, S., White, J. A., Kolb, J. F., Stacey, M. W. and Schoenbach, K. H. (2007). Compact, Nanosecond, High Repetition-Rate, Pulse Generator for Bioelectric Studies. IEEE Trans. Dielectrics and Electrical Insulation 14 (4).

Creswick, H. A.., Stacey, M. W., Kelly, Jr., R. E.., Gustin, T., Nuss, D., Harvey, H., Goretsky, M. J.., Vasser, E., Welch, J. C.., Mitchell, K. and Proud, V. K.. (2006). Family study of the inheritance of pectus excavatum. Journal of Pediatric Surgery 41 (10) , pp. 1699-703.

Sutton, J. F.., Stacey, M. W., Kearns, W. G.., Rieg, T. S.., Young, N. S.. and Liu, J. M.. (2004). Increased risk for aplastic anemia and myelodysplastic syndrome in individuals lacking glutathione S-transferase genes. Pediatr Blood Cancer 42 (2) , pp. 122-6.

Schoenbach, K. H., Joshi, R., Kolb, J. F., Chen, N., Stacey, M. W., Blackmore, P. F.., Buescher, E. S.. and Beebe, S. J. (2004). Ultrashort electrical pulses open a new gateway into biological cells. Proceedings of the Ieee 92 (7) , pp. 1122-1137.

Stacey, M. W., Stickley, J., Fox, P., Statler, V., Schoenbach, K. H., Beebe, S. J. and Buescher, S. (2003). Differential effects in cells exposed to ultra-short, high intensity electric fields: cell survival, DNA damage, and cell cycle analysis. Mutat Res 542 (1-2) , pp. 65-75.

Watters, A. D.., Stacey, M. W. and Bartlett, J. (2002). A modified nick translation method used with FISH that produces reliable results with archival tissue sections. Molecular Biotechnology 20 (3) , pp. 257-60.

Aridgides, L. J.., Stacey, M. W., Brihn, L., Scott, D. and Osgood, C. J. (2002). Fluorescence in situ hybridization on sperm using alkaline denaturation. Biotechniques 33 (2) , pp. 266-7.

Watters, A. D.., Stacey, M. W., Going, J. J.., Grigor, K. M.., Cooke, T. G.., Sim, E. and Bartlett, J. M. S.. (2001). Genetic aberrations of NAT2 and chromosome 8: Their association with progression in transitional cell carcinoma of the urinary bladder. Urologia Internationalis 67 (3) , pp. 235-239.

Pfeffer, J., Pang, M. G.., Hoegerman, S. F.., Osgood, C. J., Stacey, M. W., Mayer, J., Oehninger, S. and Kearns, W. G.. (1999). Aneuploidy frequencies in semen fractions from ten oligoasthenoteratozoospermic patients donating sperm for intracytoplasmic sperm injection. Fertility and Sterility 72 (3) , pp. 472-478.

Stacey, M. W., Matas, N., Drake, M., Payton, M., Fakis, G., Greenland, J. and Sim, E. (1999). Arylamine N-acetyltransferase type 2 (NAT2), chromosome 8 aneuploidy, and identification of a novel NAT1 cosmid clone: an investigation in bladder cancer by interphase FISH. Genes Chromosomes Cancer 25 (4) , pp. 376-83.

Thygesen, P., Risch, A., Stacey, M. W., Fakis, G., Takle, L., Knowles, M. and Sim, E. (1999). Genes for human arylamine N-acetyltransferases in relation to loss of the short arm of chromosome 8 in bladder cancer. Pharmacogenetics 9 (1) , pp. 1-8.

Stacey, M. W., Wang, J., Byrd, R. L.., Liu, J. M.. and Kearns, W. G.. (1999). Nuclear receptor co-repressor gene localizes to 17p11.2, a frequently deleted band in malignant disorders. Genes Chromosomes Cancer 25 (2) , pp. 191-3.

Stacey, M. W., Payton, M., Fakis, G., Pope, J. and Sim, E. (1998). Characterisation of arylamine N-acetyltransferase in the urothelial cell line RT112. Pathogenesis 1 (2) , pp. 99-105.

Watters, A. D.., Stacey, M. W., Going, J. J.., Grigor, K. M.., Cooke, T. G.., Sim, E. and Barlett, J. M. S.. (1998). Genetic alterations of N-acetyl transferase in transitional cell carcinoma of the bladder. British Journal of Cancer 78 (2) , pp. 154-154.

Stacey, M. W., Barlow, A. and Hulten, M. (1997). Human T-cell receptor zeta chain gene Map position 1q23.1. Chromosome Res 5 (4) , pp. 279.

Matas, N., Thygesen, P., Stacey, M. W., Risch, A. and Sim, E. (1997). Mapping AAC1, AAC2 and AACP, the genes for arylamine N-acetyltransferases, carcinogen metabolising enzymes on human chromosome 8p22, a region frequently deleted in tumours. Cytogenet Cell Genet 77 (3-4) , pp. 290-5.

Metcalfe, J. A.., Parkhill, J., Campbell, L., Stacey, M. W., Biggs, P., Byrd, P. J.. and Taylor, A. M.. (1996). Accelerated telomere shortening in ataxia telangiectasia. Nat Genet 13 (3) , pp. 350-3.

Stacey, M. W., Thygesen, P., Stanley, L., Matas, N., Risch, A. and Sim, E. (1996). Arylamine N-acetyltransferase as a potential biomarker in bladder cancer: Fluorescent in situ hybridization and immunohistochemistry studies. Biomarkers 1 (1) , pp. 55-61.

Hulten, M. A.., Stacey, M. W. and Armstrong, S. J.. (1995). Does junk DNA regulate gene expression in humans?. Clin Mol Pathol 48 (3) , pp. M118-23.

Stacey, M. W., Bennett, M. S.. and Hulten, M. (1995). FISH analysis on spontaneously arising micronuclei in the ICF syndrome. J Med Genet 32 (7) , pp. 502-8.

Hernandez, D., McConville, C. M.., Stacey, M. W., Woods, C. G.., Brown, M. M.., Shutt, P., Rysiecki, G. and Taylor, A. M.. (1993). A family showing no evidence of linkage between the ataxia telangiectasia gene and chromosome 11q22-23. J Med Genet 30 (2) , pp. 135-40.

Moss, C., Larkins, S., Stacey, M. W., Blight, A., Farndon, P. A.. and Davison, E. V.. (1993). Epidermal mosaicism and Blaschko’s lines. J Med Genet 30 (9) , pp. 752-5.

Taylor, A. M.., Lowe, P. A.., Stacey, M. W., Thick, J., Campbell, L., Beatty, D., Biggs, P. and Formstone, C. J.. (1992). Development of T-cell leukaemia in an ataxia telangiectasia patient following clonal selection in t(X;14)-containing lymphocytes. Leukemia 6 (9) , pp. 961-6.

McKeown, C. M.., Waters, J. J.., Stacey, M. W., Newman, B. F.., Cardy, D. L.. and Hulten, M. (1992). Rapid interphase FISH diagnosis of trisomy 18 on blood smears. Lancet 340 (8817) , pp. 495.

Stacey, M. W., Gallimore, P. H.., McConville, C. and Taylor, A. M.. (1990). Rearrangement of the same chromosome regions in different SV40 transformed human skin keratinocyte lines is associated with tumourigenicity. Oncogene 5 (5) , pp. 727-39.

Stacey, M. W., Thacker, S. and Taylor, A. M.. (1989). Cultured skin keratinocytes from both normal individuals and basal cell naevus syndrome patients are more resistant to gamma-rays and UV light compared with cultured skin fibroblasts. Int J Radiat Biol 56 (1) , pp. 45-58.

Taylor, A. M.., Flude, E., Laher, B., Stacey, M. W., McKay, E., Watt, J., Green, S. H.. and Harding, A. E.. (1987). Variant forms of ataxia telangiectasia. J Med Genet 24 (11) , pp. 669-77.

Books

Stacey, M. W. (2013). Detection of cytogenetic abnormalities in sperm from infertile males undergoing intracytoplasmic sperm injection. Humana Press.

Book Chapters

Kolb, J. F. and Stacey, M. W. (2011). Subcellular Biological Effects of Nanosecond Pulsed Electric Fields Plasma for Bio-decontamination, Medicine and Food Security,” NATO Science for Peace and Security Series, Springer Publishing .

Taylor, A. (1996). Malignant disease and variations in radiosensitivity in Ataxia Telangiectasia patients Genetic Predisposition to Cancer London: Chapman & Hall.

Presentations

(October 25, 2010). Am. Soc. Matrix Biol. Charleston SC.