Dreux Chappell

Ph.D. in Chemical Oceanography, Massachusetts Institute of Technology/Woods Hole Oceanographic Institution, (2009)

B.A. in Biology, Amherst College, (2000)

Expertise

Research Interests

I am a marine biogeochemist/microbial ecologist that studies the interplay between phytoplankton physiology and ocean chemistry. My research encompasses trace metal biogeochemistry, phytoplankton cultivation and physiology, and molecular marine microbiology. I use a suite of molecular biological tools and analysis of trace metal and macronutrient concentrations in seawater to link biological activity and chemical distributions in controlled laboratory settings and in the field. I study cyanobacteria and diatoms, phytoplankton that are key contributors to global biogeochemical cycles from tropical to polar environments. I focus on the development of quantitative methods to both identify marine phytoplankton in mixed assemblages and to assess functional gene expression linked to limitation by a specific element or a combination of elements and/or physical factors (co-limitation). I then use these quantified techniques to evaluate nutrient limitation in the field with the goal of better informing biogeochemical models through collaboration with modelers.Recent work has involved:-Using quantitative PCR to look at gene expression in response to changing environmental conditions (targeting functional genes)-De novo assembly of eukaryotic transcriptomesExamining community composition using fragment analysis as well as high throughput sequencing-Laboratory and field incubation experiments looking at physiological response to trace metal and macronutrient limitationI am currently looking for graduate students to join my lab group. As research projects in my laboratory tend to cross disciplines, I welcome students from a wide variety of academic backgrounds (biology, chemistry, computer science).

Articles

Mellett, T., Brown, M., Chappell, P. Dreux., Duckham, C., Fitzsimmons, J., Till, C. P., sherrell, R., Maldonado, M. and Buck, K. N. (2018). The biogeochemical cycling of iron, copper, nickel, cadmium, manganese, and scandium in a California Current experimental study. Limnol. Oceanogr. 63 (S1) , pp. S425-S447.

Nunn, S., Chappell, P. Dreux., Gomes, K., Bonderenko, A., Jenkins, B. and Nunn, B. L. (2017). Phytoplankton plastid proteomics: Cracking open diatoms to understand plastid biochemistry under iron limitation. Journal of Emerging Investigators.

Chappell, P. Dreux., Vedamati, J., Selph, K. E., Cyr, H. A., Jenkins, B. D., Landry, M. R. and Moffett, J. W. (2016). Preferential depletion of zinc within Costa Rica Upwelling Dome creates conditions for zinc co-limitation of primary production. J. Plankton Res. 38 (2) , pp. 244-255.

Chappell, P. Dreux., Whitney, L. P., Wallace, J. R., Darer, A. I., Jean-Charles, S. and Jenkins, B. D. (2014). Genetic indicators of iron limitation in wild populations of Thalassiosira oceanica from the northeast Pacific Ocean . The ISME Journal , pp. 11.

Chappell, P. Dreux., Whitney, L. P., Haddock, T. L., Menden-Deuer, S., Roy, E. G., Wells, M. L. and Jenkins, B. D. (2013). Thalassiosira spp. community composition shifts in response to chemical and physical forcing in the northeast Pacific Ocean. Frontiers in Aquatic Microbiology.

Chappell, P. Dreux., Moffett, J. W., Hynes, A. M. and Webb, E. A. (2012). Molecular Evidence of Iron Limitation and Availability in the Global Diazotroph Trichodesmium . The ISME Journal 6 , pp. 1728–1739.

Whitney, L. P., Lins, J. J., Hughes, M. P., Wells, M. L., Chappell, P. Dreux., Jenkins, B. D. and Chappell, P. Dreux. (2011). Characterization of putative iron responsive genes as species-specific indicators of iron stress in Thalassiosiroid diatoms. Frontiers in Aquatic Microbiology.

Hynes, A. M., Chappell, P. Dreux., Dyhrman, S. T., Doney, S. C. and Webb, E. A. (2009). Cross-basin comparison of phosphorus stress in Trichodesmium. Limnol. Oceanogr 54 (5) , pp. 1438-1448.

Dyhrman, S. T., Chappell, P. Dreux., Haley, S. T., Moffett, J. W., Orchard, E. D., Waterbury, J. B. and Webb, E. A. (2006). Phosphonate utilization by the globally important marine diazotroph Trichodesmium.. Nature 439 , pp. 68-71.

Conference Proceeding

Dupont, C. L., Chappell, P. Dreux., Logares, R. and Villa-Costa, M. (2010). A hitchhiker’s guide to the new molecular toolbox for ecologists Eco-DAS VIII Symposium Proceedings ASLO.

Presentations

Chappell, P. Dreux., Buck, K. N., Abdala, Z. M., Burns, S., Powell, K., Sterling, A. and Jenkins, B. D. ( 2018). Both dissolved iron and coastal water additions to Southern Drake Passage waters stimulate similar growth responses and shifts in diatom community composition. Poster OCB Summer Meeting .

Holland, L. Z., Sterling, A. R., Buck, K. N., Chappell, P. Dreux., Bundy, R. M. and Jenkins, B. D. ( 2018). Comparison of bacteria recruited by axenic Southern Ocean diatoms under iron stress. Poster OCB Summer Meeting .

Chappell, P. Dreux., Buck, K. N., Abdala, Z. M., Burns, S., Powell, K., Sterling, A. and Jenkins, B. D. ( 2018). Both dissolved iron and coastal water additions to Southern Drake Passage waters stimulate similar growth responses and shifts in diatom community composition. Oral Presentation Ocean Sciences Meeting .

Holland, L. Z., Sterling, A. R., Buck, K. N., Chappell, P. Dreux., Bundy, R. M. and Jenkins, B. D. ( 2018). Comparison of bacteria recruited by axenic Southern Ocean diatoms under iron stress. Poster Ocean Sciences Meeting .

Abdala, Z., Einarsson, S., Powell, K., Widner, B., Bernhardt, P. and Chappell, P. Dreux. ( 2018). Diatom community composition shifts in the nitrogen-limited Mid- Atlantic Bight. Poster Ocean Sciences Meeting .

Knapp, A. N., Buck, K. N., Boiteau, R., Miranda, C., McKenna, A., Corilo, Y., Caprara, S. and Chappell, P. Dreux. ( 2018). Dissolved iron and dissolved organic nitrogen concentrations are correlated in Gulf of Mexico surface waters. Oral Presentation Ocean Sciences Meeting .

Bundy, R. M., Buck, K. N., Burns, S., Sterling, A. R., Holland, L. Z., Repeta, D., Chappell, P. Dreux. and Jenkins, B. D. ( 2018). Dynamic cycling of siderophores during the growth and decline of an iron-limited phytoplankton assemblage in a Southern Ocean mesocosm experiment. Oral Presentation Ocean Sciences Meeting .

Einarsson, S., Abdala, Z., Powell, K., Till, C. P. and Chappell, P. Dreux. ( 2018). High and low iron upwelling and corresponding shifts in the diatom community. Oral Presentation Ocean Sciences Meeting .

Gomes, K. N., Holland, L. Z., Burns, S., Bundy, R. M., Buck, K. N., Chappell, P. Dreux. and Jenkins, B. D. ( 2018). Metatranscriptomic analysis of plastid targeted genes in ironlimited Southern Ocean diatoms. Poster Ocean Sciences Meeting .

Mulholland, M. R., Bernhardt, P. W., Hyde, K., Mannino, A., Widner, B., Chappell, P. Dreux. and Selden, C. ( 2018). Primary productivity and dinitrogen fixation on the North Atlantic continental shelf. Oral Presentation Ocean Sciences Meeting .

Selden, C., Chappell, P. Dreux., Widner, B., Tapia, A. M., Bernhardt, P. W. and Mulholland, M. R. ( 2018). Reconsidering coastal diazotrophy in the North Atlantic: high rates of N2 fixation along the U.S. southeastern seaboard. Oral Presentation Ocean Sciences Meeting .

Sterling, A. R., Holland, L. Z., McDermith, E. J., Buck, K. N., Chappell, P. Dreux., Bundy, R. M. and Jenkins, B. D. ( 2018). Siderophore-producing bacteria associated with diatoms in the iron-limited Southern Ocean. Poster Ocean Sciences Meeting .

Chappell, P. Dreux., Powell, K. E. and Fitzsimmons, J. N. ( 2017). Does size matter? Evaluating the bioavailability of Fe size fractions to phytoplankton field populations. Poster Chemical Oceanography Gordon Conference .

Knapp, A. N., Buck, K. N., Boiteau, R., Miranda, C., McKenna, A., Corilo, Y., Caprara, S. and Chappell, P. Dreux. ( 2017). Novel observations of correlated dissolved organic nitrogen and dissolved iron concentrations in the Gulf of Mexico. Poster Chemical Oceanography Gordon Research Conference .

Chappell, P. Dreux., Wallace, J. R., Whitney, L. P., Jenkins, B. D. and Powell, K. E. ( 2017). Ask the diatoms, using molecular methods to identify diatoms and determine their iron status. Oral Presentation Florida State University .

Abdala, Z., Einarsson, S., Powell, K., Widner, B., Bernhardt, P. and Chappell, P. Dreux. ( 2017). Diatom community composition shifts in the nitrogen limited Mid- Atlantic Bight. Poster OCB Summer Meeting .

Einarsson, S., Abdala, Z., Powell, K. and Chappell, P. Dreux. ( 2017). Diatom community composition through upwelling over thin and broad continental shelves. Poster OCB Summer Meeting .

Gomes, K. M., Nunn, B. L., Chappell, P. Dreux. and Jenkins, B. D. ( 2017). Subcellular proteomics for determining Fe-limited remodeling of plastids in the centric diatom Thalassiosira pseudonana. Poster OCB Summer Workshop .

Chappell, P. Dreux., Wallace, J. R., Whitney, L. P., Jenkins, B. D. and Powell, K. E. ( 2017). Ask the diatoms, using molecular methods to identify diatoms and determine their iron status. Oral Presentation Texas A&M University .

Jenkins, B. D., Chappell, P. Dreux., Whitney, L. P., Wallace, J. R. and Gomes, K. ( 2016). Transcriptome profiling for elucidating nutritional ecology in diatoms. Oral Presentation EMBO/EMBL Symposium .

Chappell, P. Dreux., Wallace, J. R. and Jenkins, B. D. ( 2016). Using comparative transcriptomics and database mining to develop molecular markers for iron limitation in diatoms. Poster EMBO/EMBL Symposium A New Age of Discovery for Aquatic Microeukaryotes .

Einarsson, S., Abdala, Z., Powell, K., Twining, B., Till, C. P., Coale, T. and Chappell, P. Dreux. ( 2016). Diatom community composition along Oregon Coast upwelling in relation to environmental variables. Poster OCB Summer Workshop .

Abdala, Z., Einarsson, S., Powell, K., Fitzsimmons, J., Coale, T., Till, C. P. and Chappell, P. Dreux. ( 2016). Diatom community composition shifts as a response to California coastal upwelling. Poster OCB Summer Workshop .

Chappell, P. Dreux. ( 2016). Molecular indicators of trace element bioavailability Oral Presentation OCB/GEOTRACES Internal Cycling Workshop .

Abdala, Z. M., Powell, K., Cronin, D. P. and Chappell, P. Dreux. ( 2016). A comparative gene expression analysis of iron-limited cultures of Chaetoceros socialis and Pseudo-nitzschia arenysensisusing newly developed iron assays. Poster Ocean Sciences Meeting .

Chappell, P. Dreux., Wallace, J., Jenkins, B. D. and Powell, K. ( 2016). Combining ‘OMIC tools and other targeted molecular methods to evaluate iron limitation of diatoms in the Northeast Pacific Ocean. Oral Presentation Ocean Sciences Meeting .

Chappell, P. Dreux., Fitzsimmons, J. N., Ohnemus, D., Powell, K. E. and Twining, B. ( 2015). Testing bioavailability of different iron sources using incubations and gene expression analyses. Oral Presentation IRN-BRU Workshop .

Jenkins, B. D., Wallace, J. R., Chappell, P. Dreux. and Gomes, K. ( 2015). Transcriptome profiling for elucidating nutritional ecology in diatoms. Oral Presentation Molecular Life of Diatoms Meeting .

Chappell, P. Dreux., Wallace, J. R., Whitney, L. P., Jenkins, B. D. and Powell, K. E. ( 2015). Developing the molecular toolkit to study linkages between ocean chemistry and diatom physiology. Oral Presentation University of Maine .

Jenkins, B. D., Chappell, P. Dreux., Whitney, L. P., Wallace, J. R. and Leigh, T. N. ( 2014). Diatoms living in different iron regimes in the North Pacific Ocean. Oral Presentation Goldschmidt Conference .

Jenkins, B. D. and Chappell, P. Dreux. ( 2014). A new method for following diatom taxonomy to enable genome-based studies in the environment. Oral Presentation Joint Aquatic Sciences Meeting .

Chappell, P. Dreux., Moffett, J. W., Cyr, H. A., Vedamati, J. and Jenkins, B. D. ( 2014). Trace metal-silicate co-limitation of diatoms in the Costa Rica Dome. Poster Ocean Sciences Meeting .

Chappell, P. Dreux. ( 2014). Developing the molecular toolkit to identify diatoms and determine their iron status in the field Oral Presentation Old Dominion University .

Chappell, P. Dreux. ( 2014). Using the CLC Genomics Workbench on Turing to Study Global Gene Expression in Non-Model Organisms Oral Presentation Old Dominion University HPC Day .

Jenkins, B. D. and Chappell, P. Dreux. (May 22, 2014). A New Method for Following Diatom Taxonomy to Enable Genome-based Studies in the Environment. Oral Presentation Joint Aquatic Sciences Meeting Portland, OR.

Chappell, P. Dreux. and Jenkins, B. D. (May 22, 2014). Comparison of whole cell transcriptional response to light and Fe in multiple diatom species. Oral Presentation Joint Aquatic Sciences Meeting Portland, OR.

Degree, A., Chappell, P. Dreux., Hill, V. and Zimmerman, R. C. (February 25, 2014). Optical Analysis of Iron Deplete and Replete Diatom Pigments. Poster Ocean Sciences 2014 Honolulu, HI.

Jenkins, B. D., Chappell, P. Dreux., Wallace, J. R. and Whitney, L. P. (February 24, 2014). Following Iron Limitation Across Ocean Gradients: Pairing Genetic Fingerprinting of Diatom Community Composition with Molecular Indicators of Iron Status. Oral Presentation Ocean Sciences 2014 Honolulu, HI.

Chappell, P. Dreux., Moffett, J. W., Cyr, H. A., Vedamati, J. and Jenkins, B. D. (February 24, 2014). Trace Metal-Silicate CO-Limitation of Diatoms in the Costa Rica Dome. Poster Ocean Sciences 2014 Honolulu, HI.

Chappell, P. Dreux., Whitney, L. P., Maness, S. L., Vedamati, J., Moffett, J. W. and Jenkins, B. D. (February 22, 2013). Profiling Thalassiosira Community Composition and Iron Status on the Geomics Cruise using Molecular Methods. Oral Presentation Aquatic Sciences Meeting New Orleans, LA.

Whitney, L. P., Chappell, P. Dreux. and Jenkins, B. D. (February 21, 2013). Using Molecular Tools to Assess the Response to FR Available in the Diatom Thalassiosira Oceanica from the Northeast Pacific. Poster Aquatic Sciences Meeting New Orleans, LA.

Wallace, J. R., Jenkins, B. D. and Chappell, P. Dreux. (February 19, 2013). Profiling Nutrient Limitation in Thalassiosiroid Diatoms via Global Gene Expression Analysis. Poster Aquatic Sciences Meeting New Orleans, LA.

Chappell, P. Dreux., Whitney, L. P. and Jenkins, B. D. ( 2012). Transcriptome of iron limited Thalassiosira oceanica provides insight into the iron stress response of oceanic diatoms. Poster Marine Microbes Gordon Research Conference .

Chappell, P. Dreux. ( 2012). Sleuthing the Sea: Developing molecular tools to link ocean chemistry and phytoplankton physiology. Oral Presentation Old Dominion University .

Chappell, P. Dreux. ( 2012). Sleuthing the Sea: Developing molecular tools to link ocean chemistry and phytoplankton physiology. Oral Presentation University of South Florida .

Chappell, P. Dreux. and Jenkins, B. D. ( 2011). Thalassiosirid barcoding method reveals coastal diversity and regions of single species dominance in the Eastern Subarctic Pacific Ocean. Oral Presentation ASLO Meeting .

Chappell, P. Dreux., Darer, A., Whitney, L. P., Haddock, T. L., Lins, J. J., Roy, E., Wells, M. and Jenkins, B. D. ( 2011). Combining fragment analysis and qRT-PCR to evaluate iron limitation of Thalassiosirids in the eastern subarctic Pacific Ocean. Poster Chemical Oceanography Gordon Research Conference .

Darer, A., Chappell, P. Dreux. and Jenkins, B. D. ( 2011). A gene-based indicator of iron limitation in the diatom Thalassiosira oceanica. Poster Ocean Sciences Meeting .

Jenkins, B. D., Rynearson, T. A., Dyrhman, S. T., Saito, M. A., Chappell, P. Dreux. and Whitney, L. P. ( 2011). From lab to launch: integrating biomarkers derived from genomics and proteomics approaches into remote observing platforms. Oral Presentation Ocean Sciences Meeting .

Chappell, P. Dreux. ( 2011). The Microbial Whodunit?: Developing molecular biological tools to link ocean chemistry and microbial activity. Oral Presentation RSMAS (U Miami) .

Jenkins, B. D., Chappell, P. Dreux., Mercier, M., Rynearson, T. A., Pritchard, L. B., Lins, J. J., Dyhrman, S. and Saito, M. A. ( 2010). Following the complete cellular response to iron limitation in diatoms. Poster Marine Microbes Gordon Conference .

Chappell, P. Dreux., Lins, J. J., Ventura, J. and Jenkins, B. D. ( 2010). Developing Genomic and Proteomic Infrastructure to Study the Diatom Genus Thalassiosira in the Laboratory and the Field. Poster Ocean Sciences Meeting .

Chappell, P. Dreux., Moffett, J. W., Hynes, A. M., Waterbury, J. and Webb, E. A. ( 2009). Development and Implementation of an Fe Stress Assay in Trichodesmium spp. Oral Presentation Aquatic Sciences Meeting .

Chappell, P. Dreux. ( 2009). The Relationship Between Iron and Nitrogen Fixation in Trichodesmium spp. Poster Chemical Oceanography Gordon Research Conference .

Chappell, P. Dreux., Hynes, A. M., Moffett, J. W., Waterbury, J. and Webb, E. A. ( 2009). Demonstration of iron limitation of N2 fixation in the lab and the field and implications for iron availability in Trichodesmium. Oral Presentation URI Graduate School of Oceanography Bio at Noon Seminar .

Chappell, P. Dreux., Moffett, J. W., Waterbury, J. and Webb, E. A. ( 2008). Development and Implementation of an Fe Stress Assay in Trichodesmium spp. Oral Presentation Dissertations Symposium on Chemical Oceanography .

Chappell, P. Dreux. and Hynes, A. M. ( 2008). Diversity and Iron Ecology of Trichodesmium. Oral Presentation Ecological Dissertations in the Aquatic Sciences .

Chappell, P. Dreux., Moffett, J. W., Waterbury, J. and Webb, E. A. ( 2008). Using QRT-PCR of the Fe Stress Response Gene isiB to Relate N Fixation Rates and Growth Under Different Fe Conditions in Trichodesmium spp. Poster Ocean Sciences Meeting .

Chappell, P. Dreux., Moffett, J. W., Waterbury, J. and Webb, E. A. ( 2008). Using QRT-PCR of the Fe Stress Response Gene isiB to Relate N Fixation Rates and Growth Under Different Fe Conditions in Trichodesmium spp. Poster Southern California Geobiology Symposium .

Chappell, P. Dreux., Moffett, J. W., Waterbury, J. and Webb, E. A. ( 2007). The Trichodesmium Fe Stress Response: Development of A Field Assay to Link Gene Expression and Nitrogen Fixation Rates. Oral Presentation EGGS Seminar Princeton University .

Chappell, P. Dreux., Moffett, J. W., Waterbury, J. and Webb, E. A. ( 2007). Exploring the Linkages between Fe Levels, Nitrogen Fixation Rates and the Expression of Fe Stress Response Genes in Trichodesmium. Oral Presentation The Center for Environmental BioInorganic Chemistry (CEBIC) Summer Conference, Princeton University .

Dyhrman, S., Chappell, P. Dreux., Haley, S., Moffett, J. W., Orchard, E., Waterbury, J. and Webb, E. ( 2006). Molecular evidence of phosphonate utilization in Trichodesmium. Oral Presentation Ocean Sciences Meeting .

Chappell, P. Dreux., Moffett, J. W., Haley, S., Dyhrman, S. and Webb, E. A. ( 2006). Using Gene Expression to Evaluate P and Fe Stress in Sargasso Sea Populations of Trichodesmium. Oral Presentation Ocean Sciences Meeting .

Chappell, P. Dreux., Moffett, J. W. and Webb, E. A. ( 2005). Evaluation of idiA and feoB Gene Expression as Markers of Iron Stress in Trichodesmium. Oral Presentation ASLO Aquatic Sciences Meeting .

Chappell, P. Dreux., Moffett, J. W. and Webb, E. A. ( 2004). Characterization of Two Genes Involved in Iron Transport in Trichodesmium. Poster Environmental Bioinorganic Chemistry Gordon Research Conference .

• 2017: ODU College of Sciences Early Career Distinguished Faculty Award, College of Sciences, Old Dominion University
• 2008: Selected to participate in DISCO XXI Symposium,
• 2008: Selected to participate in Eco-DAS VIII Symposium,
• 2005: EPA Star Fellowship (Declined),
• 2005: National Defense Science and Engineering Graduate (NDSEG) Fellowship,
• 2005: NSF Graduate Research Fellowship Honorable Mention,
• 2000: High Honors in Biology, Amherst College